The gains in resolution, contrast, and optical sectioning enable imaging in low-SNR conditions and single-molecule tracking in living fruit fly embryos, revealing how individual transcripts diffuse in their intracellular environment

In complex multicellular systems, like organoids and gastruloids, resolution is often limited by scattering-induced out-of-focus background, not diffraction.

2P-OPM suppresses this background, maintains subcellular resolution at depth, and works with standard sample mounting

New preprint 📰

We present 2P-OPM: a microscope that combines high-NA single-objective detection with two-photon plane illumination to improve spatial resolution at depth

arxiv.org/abs/2511.09462