Time to say 'Au revoir Paris' after a fantastic #KLEBS2024. Great science and great people. So proud to have @tomstantonmicro.bsky.social @kalanip.bsky.social @bananabenana.bsky.social representing the lab, and congrats to Tom and Kalani for their poster prizes! 👏

Here we go! #KLEBS2024
400 in person and 400 online participants from 71 countries...... Wow!

When we tested some of these clone-specific core substrates in the lab, we found evidence of reciprocal cross-feeding between clones!

When we took a close look at 48 common clones (sub-lineages) we found each had a unique predicted phenotype profile.

We defined clone-specific core phenotypes- some common to many clones, some rarer e.g. global MDR SLs 258 and 307 could use fructoselysine, but SL147 couldn't

But there was variation within species too!

This was structured so that more closely related isolates shared more similar sets of metabolic traits.

We simulated 1300 growth phenotypes for each isolate (different carbon, nitrogen, phosphorus, sulfur sources in minimal media +/- O2).

Phenotype profiles differed by species - as we expected - and were generally consistent with formal species definitions.

We used a combination of genomics and metabolic modelling of >7K Klebs pneumo complex isolates to explore diversity and distribution of metabolic traits.

Most individual traits are either core or very rare - just like genes in the total pan-genome.

and predict growth phenotypes with high accuracy in both aerobic and anaerobic conditions

The model can be used in conjunctive with Bactabolize (github.com/kelwyres/Bac...) to rapidly derive strain-specific metabolic models for K. pneumoniae and its close relatives. These models capture and simulate greater metabolic diversity than those derived from other references.

Back to the socials after the holidays and first post is an absolute joy-->
📣📣📣 Congratulations to twitter.com/helena_betha... for preprinting the first chapter of her PhD thesis 🎉🎉🎉

doi.org/10.1101/2023...