Julian Kompa
@juliankompa.bsky.social
PhD student in the group of Kai Johnsson, Chemical Biology department of Max Planck Institute for Medical Research Heidelberg | Interested in labeling strategies for super-resolution microscopy
Reposted by Julian Kompa
Nature Methods is also hiring to replace me (applications close August 4th!!!) and I will do everything I can to support their next bioimaging editor (ask me anything!). springernature.wd3.myworkdayjobs.com/SpringerNatu...
Associate Editor or Senior Editor, Nature Methods
Title: Associate or Senior Editor, Nature Methods Organization: Nature Portfolio Locations: New York, Jersey City, Shanghai or Beijing Closing Date: August 3, 2025 About Springer Nature Springer Na...
springernature.wd3.myworkdayjobs.com
August 1, 2025 at 2:27 PM
Nature Methods is also hiring to replace me (applications close August 4th!!!) and I will do everything I can to support their next bioimaging editor (ask me anything!). springernature.wd3.myworkdayjobs.com/SpringerNatu...
We propose the elimination of thioethers as a general strategy to enhance the photostability of self-labeling protein tags. Happy that we could support this mechanism with a MS analysis. The M175L mutation of HaloTag yields up to 4-fold more photons. 🎇 (4/4)
www.pnas.org/doi/abs/10.1...
www.pnas.org/doi/abs/10.1...
Thioether editing generally increases the photostability of rhodamine dyes on self-labeling tags | PNAS
Self-labeling protein tags are widely used in advanced bioimaging where dyes with
high-photon budgets outperform their fluorescent protein counterp...
www.pnas.org
July 29, 2025 at 7:24 AM
We propose the elimination of thioethers as a general strategy to enhance the photostability of self-labeling protein tags. Happy that we could support this mechanism with a MS analysis. The M175L mutation of HaloTag yields up to 4-fold more photons. 🎇 (4/4)
www.pnas.org/doi/abs/10.1...
www.pnas.org/doi/abs/10.1...
Kinprola is based on split-HaloTag and records the activity of, for instance, PKA via chemical labeling in live cells. 📈
The kinase activity is reflected by the accumulation of a permanent fluorescent signal and can be correlated with transcriptomics. (3/4)
www.nature.com/articles/s41...
The kinase activity is reflected by the accumulation of a permanent fluorescent signal and can be correlated with transcriptomics. (3/4)
www.nature.com/articles/s41...
Molecular recording of cellular protein kinase activity with chemical labeling - Nature Chemical Biology
Molecular recorders based on kinase activity-dependent protein labeling track specific kinase activities to understand their link to cellular phenotypes in heterogeneous cell populations and in vivo.
www.nature.com
July 29, 2025 at 7:24 AM
Kinprola is based on split-HaloTag and records the activity of, for instance, PKA via chemical labeling in live cells. 📈
The kinase activity is reflected by the accumulation of a permanent fluorescent signal and can be correlated with transcriptomics. (3/4)
www.nature.com/articles/s41...
The kinase activity is reflected by the accumulation of a permanent fluorescent signal and can be correlated with transcriptomics. (3/4)
www.nature.com/articles/s41...
SNAP-tag2, combined with its novel substrate TF, enabels faster and brighter protein labeling with synthetic dyes. 💡 Thereby we close the performance gap to HaloTag, making SNAP-tag more attractive for super-resolution microscopy. (2/4)
www.nature.com/articles/s41...
www.nature.com/articles/s41...
SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology
SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...
www.nature.com
July 29, 2025 at 7:24 AM
SNAP-tag2, combined with its novel substrate TF, enabels faster and brighter protein labeling with synthetic dyes. 💡 Thereby we close the performance gap to HaloTag, making SNAP-tag more attractive for super-resolution microscopy. (2/4)
www.nature.com/articles/s41...
www.nature.com/articles/s41...