Jovana Kaljevic ⬜️
@jovanakaljevic.bsky.social
Postdoctoral scientist | JIC, UK
PhD | de Duve institute, Belgium
Passionate about killing E. coli and watching them die under the🔬
PhD | de Duve institute, Belgium
Passionate about killing E. coli and watching them die under the🔬
Reposted by Jovana Kaljevic ⬜️
Happy to finally show our story of the connection between nucleoid conformation, transcription & predation in our favorite predator Bdellovibrio bacteriovorus. 🧵 1/n
📝New preprint! #MicroSky
🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.
🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.
www.biorxiv.org/content/10.1...
🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.
🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.
www.biorxiv.org/content/10.1...
Dynamics and interplay of gene expression and chromosome organization across a predatory lifecycle
The obligate predatory bacterium Bdellovibrio bacteriovorus alternates between a motile attack phase and a growth phase inside another bacterium, during which it undergoes multiple rounds of DNA repli...
www.biorxiv.org
September 26, 2025 at 9:09 AM
Happy to finally show our story of the connection between nucleoid conformation, transcription & predation in our favorite predator Bdellovibrio bacteriovorus. 🧵 1/n
Thrilled to see this work out. I enjoyed our collaboration @antoinehocher and loved working on this project. Congrats to the entire team! @tunglejic.bsky.social
new preprint from our group & Antoine Hocher: www.biorxiv.org/content/10.1...
A fantastic collaboration with Antoine, with Jovana Kaljevic' initiated the collaboration and drives the project.
A fantastic collaboration with Antoine, with Jovana Kaljevic' initiated the collaboration and drives the project.
Versatile NTP recognition and domain fusions expand the functional repertoire of the ParB-CTPase fold beyond chromosome segregation
Nucleotide triphosphate (NTP)-dependent molecular switches regulate essential cellular processes by cycling between active and inactive states through nucleotide binding and hydrolysis. These mechanis...
www.biorxiv.org
October 11, 2025 at 1:12 PM
Thrilled to see this work out. I enjoyed our collaboration @antoinehocher and loved working on this project. Congrats to the entire team! @tunglejic.bsky.social
Reposted by Jovana Kaljevic ⬜️
ParB NTPase fold might:
*suggest hidden layer of biological regulation
*maybe a fold as fundamental as P-loop ATPases or Rossmann folds (well, this has to be seen!)
*be a treasure trove of uncharacterized proteins waiting to be studied.
We probably have only scratched the surface.
*suggest hidden layer of biological regulation
*maybe a fold as fundamental as P-loop ATPases or Rossmann folds (well, this has to be seen!)
*be a treasure trove of uncharacterized proteins waiting to be studied.
We probably have only scratched the surface.
October 11, 2025 at 11:36 AM
ParB NTPase fold might:
*suggest hidden layer of biological regulation
*maybe a fold as fundamental as P-loop ATPases or Rossmann folds (well, this has to be seen!)
*be a treasure trove of uncharacterized proteins waiting to be studied.
We probably have only scratched the surface.
*suggest hidden layer of biological regulation
*maybe a fold as fundamental as P-loop ATPases or Rossmann folds (well, this has to be seen!)
*be a treasure trove of uncharacterized proteins waiting to be studied.
We probably have only scratched the surface.
This was fun!
🚨New paper out! #MicroSky
Studying obligate predators like Bdellovibrio bacteriovorus is tricky—essential genes for predation are also essential for survival.
We expanded its genetic toolbox:
🧬promoters to fine-tune expression
🧬IPTG-inducible system
🧬CRISPRi for rapid knockdown
bit.ly/46GUn2c
1/4
Studying obligate predators like Bdellovibrio bacteriovorus is tricky—essential genes for predation are also essential for survival.
We expanded its genetic toolbox:
🧬promoters to fine-tune expression
🧬IPTG-inducible system
🧬CRISPRi for rapid knockdown
bit.ly/46GUn2c
1/4
Expanding the genetic toolbox of the obligate predatory bacterium Bdellovibrio bacteriovorus with inducible gene expression and CRISPR interference
Inducible gene expression and CRISPR interference-mediated depletion in the predatory bacterium Bdellovibrio bacteriovorus.
academic.oup.com
October 6, 2025 at 8:44 PM
This was fun!
Reposted by Jovana Kaljevic ⬜️
📝New preprint! #MicroSky
🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.
🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.
www.biorxiv.org/content/10.1...
🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.
🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.
www.biorxiv.org/content/10.1...
Dynamics and interplay of gene expression and chromosome organization across a predatory lifecycle
The obligate predatory bacterium Bdellovibrio bacteriovorus alternates between a motile attack phase and a growth phase inside another bacterium, during which it undergoes multiple rounds of DNA repli...
www.biorxiv.org
September 25, 2025 at 7:12 AM
📝New preprint! #MicroSky
🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.
🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.
www.biorxiv.org/content/10.1...
🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.
🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.
www.biorxiv.org/content/10.1...
doi.org/10.1038/d41586…
Check out our story! From inseparable undergrads in Belgrade to PhDs scattered across the globe, FFDS (Four Friends Doing Science) kept us inspiring each other, no matter the distance.
Can you guess who’s who😆?
@BorisStojilkovic @milicajelicic
@tismaa.bsky.social
Check out our story! From inseparable undergrads in Belgrade to PhDs scattered across the globe, FFDS (Four Friends Doing Science) kept us inspiring each other, no matter the distance.
Can you guess who’s who😆?
@BorisStojilkovic @milicajelicic
@tismaa.bsky.social
November 28, 2024 at 6:14 PM
doi.org/10.1038/d41586…
Check out our story! From inseparable undergrads in Belgrade to PhDs scattered across the globe, FFDS (Four Friends Doing Science) kept us inspiring each other, no matter the distance.
Can you guess who’s who😆?
@BorisStojilkovic @milicajelicic
@tismaa.bsky.social
Check out our story! From inseparable undergrads in Belgrade to PhDs scattered across the globe, FFDS (Four Friends Doing Science) kept us inspiring each other, no matter the distance.
Can you guess who’s who😆?
@BorisStojilkovic @milicajelicic
@tismaa.bsky.social