John Ngo
@johntngo.bsky.social
Associate Prof of BME at BU
Synthetic mechanobiology, single molecules, molecular structure and engineering, bio-organic chemistry, and general enthusiast of science, kindness, and understanding.
www.thengolab.com
Synthetic mechanobiology, single molecules, molecular structure and engineering, bio-organic chemistry, and general enthusiast of science, kindness, and understanding.
www.thengolab.com
Ah yes... "Non-Canonical Amino Acids"... the "accents" of life's alphabet... So hot right now! 🧑🔬
November 11, 2025 at 1:12 AM
Ah yes... "Non-Canonical Amino Acids"... the "accents" of life's alphabet... So hot right now! 🧑🔬
Such a talent! And clearly, one is expressing GFP...
October 22, 2025 at 2:10 AM
Such a talent! And clearly, one is expressing GFP...
Glad you commented! Here’s the ReadCube link! rdcu.be/eKLv5
Synthetic mechanotransduction
Nature Reviews Bioengineering - Cells convert mechanical forces into biochemical signals through mechanotransduction, a process which can now be synthetically engineered. In this Review, we examine...
rdcu.be
October 15, 2025 at 8:46 PM
Glad you commented! Here’s the ReadCube link! rdcu.be/eKLv5
Wow—thank you so much Evelina! We have long been inspired by your work! Please let us know what you see (and let us know if we can help!). 😎
September 23, 2025 at 1:41 PM
Wow—thank you so much Evelina! We have long been inspired by your work! Please let us know what you see (and let us know if we can help!). 😎
Many thanks, Gal!!! We’re excited to see the paper live online! =)
September 22, 2025 at 9:28 PM
Many thanks, Gal!!! We’re excited to see the paper live online! =)
Thanks Brian! it was a a fun project led by two super talented students. Glad you like the movies! 😎
September 22, 2025 at 6:54 PM
Thanks Brian! it was a a fun project led by two super talented students. Glad you like the movies! 😎
Thanks Dan! We hope so. Plasmids are on AddGene… let us know what you see!
September 22, 2025 at 6:52 PM
Thanks Dan! We hope so. Plasmids are on AddGene… let us know what you see!
3️⃣ Future synthetic biology utilities:
Right now, we are fusing these proteins to #synbio effectors and adaptors to:
• control RNA fate,
• boost CRISPR/dCas9 RNA scaffolds,
• reduce off-target labeling
enabling next-gen RNA–protein circuits for synthetic regulation and control.
Right now, we are fusing these proteins to #synbio effectors and adaptors to:
• control RNA fate,
• boost CRISPR/dCas9 RNA scaffolds,
• reduce off-target labeling
enabling next-gen RNA–protein circuits for synthetic regulation and control.
September 22, 2025 at 6:27 PM
3️⃣ Future synthetic biology utilities:
Right now, we are fusing these proteins to #synbio effectors and adaptors to:
• control RNA fate,
• boost CRISPR/dCas9 RNA scaffolds,
• reduce off-target labeling
enabling next-gen RNA–protein circuits for synthetic regulation and control.
Right now, we are fusing these proteins to #synbio effectors and adaptors to:
• control RNA fate,
• boost CRISPR/dCas9 RNA scaffolds,
• reduce off-target labeling
enabling next-gen RNA–protein circuits for synthetic regulation and control.
2️⃣ Advantages in Imaging & Controlling Single mRNAs:
• Single-mRNA sensitivity using 1×mNeonGreen fusions in live #imaging
• Track the localization & translation #mRNAs encoding cytoplasmic, nuclear, mitochondrial & secreted proteins
• Direct #mRNA localization by fusing dMCP to targeting signals
• Single-mRNA sensitivity using 1×mNeonGreen fusions in live #imaging
• Track the localization & translation #mRNAs encoding cytoplasmic, nuclear, mitochondrial & secreted proteins
• Direct #mRNA localization by fusing dMCP to targeting signals
September 22, 2025 at 6:27 PM
1️⃣ Mechanism & protein engineering:
Through circular permutation + degron masking, we designd dMCP & dPCP to be rapidly degraded unless bound to their cognate #RNA hairpins (MS2 & PP7). Hairpin binding switches them from unstable to bright, RNA-bound reporters via a #degron masking mechanism.
Through circular permutation + degron masking, we designd dMCP & dPCP to be rapidly degraded unless bound to their cognate #RNA hairpins (MS2 & PP7). Hairpin binding switches them from unstable to bright, RNA-bound reporters via a #degron masking mechanism.
September 22, 2025 at 6:27 PM
Congratulations, Rita!! And thank you for all the awesome work you do. Nature Biomedical Engineering is lucky to have you at the helm!
August 1, 2025 at 4:01 PM
Congratulations, Rita!! And thank you for all the awesome work you do. Nature Biomedical Engineering is lucky to have you at the helm!
A world without science is one without hope and progress!
July 17, 2025 at 4:20 AM
A world without science is one without hope and progress!