John Ngo
@johntngo.bsky.social
Associate Prof of BME at BU
Synthetic mechanobiology, single molecules, molecular structure and engineering, bio-organic chemistry, and general enthusiast of science, kindness, and understanding.
www.thengolab.com
Synthetic mechanobiology, single molecules, molecular structure and engineering, bio-organic chemistry, and general enthusiast of science, kindness, and understanding.
www.thengolab.com
🚀 Our new paper is out @natmethods.nature.com!
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
September 22, 2025 at 6:27 PM
🚀 Our new paper is out @natmethods.nature.com!
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
This group photo from the 1999 Bioorganic GRC is everything… and more!
June 21, 2025 at 10:48 PM
This group photo from the 1999 Bioorganic GRC is everything… and more!
Greetings from the 2025 Bioorganic GRC, where this gem is on display in the meeting history books. So many familiar faces—who do you see? Check out right side row 3 for RYT. @michaelzlin.bsky.social
June 19, 2025 at 3:27 AM
Greetings from the 2025 Bioorganic GRC, where this gem is on display in the meeting history books. So many familiar faces—who do you see? Check out right side row 3 for RYT. @michaelzlin.bsky.social
Thank you for a great semester, BE 209!!
May 9, 2025 at 9:42 PM
Thank you for a great semester, BE 209!!
Gracias, Lukasz! In case you are wondering, here’s the picture I initially proposed for this. Let’s say: ACS and I settled on a compromise! 🤓
May 1, 2025 at 4:05 AM
Gracias, Lukasz! In case you are wondering, here’s the picture I initially proposed for this. Let’s say: ACS and I settled on a compromise! 🤓
To my fellow young scientists---I have one key message to share with you right now: do what you love and love what you do. That is exactly science needs right now.
April 18, 2025 at 2:56 AM
To my fellow young scientists---I have one key message to share with you right now: do what you love and love what you do. That is exactly science needs right now.
Plasmids for our destabilized MCP (dMCP) & PCP (dPCP) coat proteins are now on @addgene.bsky.social here: www.addgene.org/browse/article/28252367/
These proteins are degraded by cells except when bound to their RNA hairpin targets (MS2 & PP7): www.biorxiv.org/content/10.1101/2024.11.21.624393v1
These proteins are degraded by cells except when bound to their RNA hairpin targets (MS2 & PP7): www.biorxiv.org/content/10.1101/2024.11.21.624393v1
March 3, 2025 at 9:09 PM
Plasmids for our destabilized MCP (dMCP) & PCP (dPCP) coat proteins are now on @addgene.bsky.social here: www.addgene.org/browse/article/28252367/
These proteins are degraded by cells except when bound to their RNA hairpin targets (MS2 & PP7): www.biorxiv.org/content/10.1101/2024.11.21.624393v1
These proteins are degraded by cells except when bound to their RNA hairpin targets (MS2 & PP7): www.biorxiv.org/content/10.1101/2024.11.21.624393v1
I like how they always find a bunch of non-proteogenic amino acids on these things (norvaline, diaminobutyric acid, etc…). Also interesting is this:
February 5, 2025 at 12:06 AM
I like how they always find a bunch of non-proteogenic amino acids on these things (norvaline, diaminobutyric acid, etc…). Also interesting is this:
In 1995, Lubert sent his good friend Roger Tsien an autographed copy of his famous Biochemistry textbook. Roger, like Lubert, also loved vibrant colors.
December 17, 2024 at 12:22 AM
In 1995, Lubert sent his good friend Roger Tsien an autographed copy of his famous Biochemistry textbook. Roger, like Lubert, also loved vibrant colors.
We designed MS2 and PP7 binding coat proteins that are degraded in cells except when bound by their hairpin RNAs.
You can check out our pre-print here: tinyurl.com/27p7hyt9,
and listen to a Google AI-generated podcast on it here: notebooklm.google.com/notebook/d71...
You can check out our pre-print here: tinyurl.com/27p7hyt9,
and listen to a Google AI-generated podcast on it here: notebooklm.google.com/notebook/d71...
November 30, 2024 at 8:48 PM
We designed MS2 and PP7 binding coat proteins that are degraded in cells except when bound by their hairpin RNAs.
You can check out our pre-print here: tinyurl.com/27p7hyt9,
and listen to a Google AI-generated podcast on it here: notebooklm.google.com/notebook/d71...
You can check out our pre-print here: tinyurl.com/27p7hyt9,
and listen to a Google AI-generated podcast on it here: notebooklm.google.com/notebook/d71...
Messages loud & clear: Chris & Alex designed destabilized MS2 and PP7 coat proteins, which are degraded in cells except when bound to their cognate RNA hairpins. We used these probes to image distinct single mRNAs simultaneously in live cells, 'background-free.' AddGene soon! tinyurl.com/27p7hyt9
November 22, 2024 at 3:39 AM
Messages loud & clear: Chris & Alex designed destabilized MS2 and PP7 coat proteins, which are degraded in cells except when bound to their cognate RNA hairpins. We used these probes to image distinct single mRNAs simultaneously in live cells, 'background-free.' AddGene soon! tinyurl.com/27p7hyt9