I finally get to join an honoured festive tradition in #DuvetKnowItsChristmas
Parents are stopping over in my 1-bed flat before we go join the family gathering in the morning. They get my bed, I get the airbed on my own living room floor.
Also featuring camping chair to supplement my 2-seat sofa

Finally, we show that interactions between the PNUTS TND and corresponding TND-interacting motifs in other proteins are also essential for transcription, and we propose that these interactions are a key substrate selection mechanism for PNUTS-PP1 to control transcription pause release (5/8)

We then use an elegant rescue system designed by @neilblackledge.bsky.social to show that the interaction between PNUTS and the phosphatase subunit PP1 is essential for transcription, and identify a number of potential substrates including Cdk9, SPT6 and the PAF complex (4/8)

In the absence of PNUTS, RNA Pol II accumulates at the 5’ end of genes and elongating polymerase is lost from gene bodies. We profile the occupancy of factors that regulate the early stages of transcription and conclude that PNUTS is required for exit from pausing into productive elongation (3/8)

First, calibrated TTseq showed that rapid PNUTS removal using the dTAG degron system results not only in massive transcription termination defects (as previously described), but also dramatic reductions in gene body transcription (2/8)