Finally in collaboration with Scott Hansen's lab we showed this nanobody blocked EFR3 mediated membrane recruitment using his single molecule TIRF approach, and with Tamas Balla's lab showed this nanobody blocked EFR3 mediated PM recruitment in cells

This nanobody clearly blocked efr3 recruitment in a dose dependent fashion

Sushant and Damilola in my group were running the BLI instrument nonstop to generate all of these curves. Alex was able to solve the cryo EM structure of this nanobody bound to PI4KA-TTC7B

This is important as diseases are caused by hyperactivation of PI4KA, but complete loss is toxic. Therefore we reasoned that we might be able to selectively target unique PI4K regulatory complexes. We developed a nanobody that was highly TTC7B selective

We fully characterised all possible regulatory complexes that can form between all 6 possible combinations of PI4KA regulatory complexes containing EFR3, TTC7 and FAM126 isoforoms

Great visit to the university of torino for a seminar, fun science and great food with good friends