Do you think this could work for small domains on larger proteins/complexes?

4/4
A big thanks to Jose Maria Carazo and Carlos Oscar Sorzano for their amazing guidance and support throughout this project and my Marie Curie fellowship generally

And thanks to MRC LMB scientific computing and funders MRC, Horizon EU and CSIC

3/4
Thanks and congratulations to all authors: Aditya Pokharna, Imogen Stockwell, Josip Ivica, Bishal Singh, Johannes Schwab, Carlos Vega-Gutierrez, Ondrej Cais, Beatriz Herguedas, and Ingo Greger

2/4
We used a combination of Cryo-EM and simulations, including classical MD and metadynamics, along with electrophysiology for kinetics and synaptic targeting

Some amino acid changes can happen with just one nucleotide change but not all of them. A 2-nucleotide mutation is one where 2 nucleotides of the three encoding that amino acid undergo changes and that’s rarer

I imagine we could have a mechanism of having pull requests that get reviewed?

It’s fine to discuss things in a constructive way. The comment of yours that I contradicted is the one where you said people don’t care

We are the scientists/professionals talking to each other. If you don’t care then don’t follow us.

Where does the specificity for cancer cells come from?

(3) This paper is now out for review again (woo!); if you have read this and received it to review - please consider writing your review in light of our response to reviewers!

(2) Further, @fraserlab.bsky.social conducted a great public peer review of this (thanks Daphne, CJ, and Jamie)! We want to respond to their comments, as well as some replies to our original reviewers. This response-to-reviewers can be found here: www.dropbox.com/scl/fi/v5iqy...

2 pore potassium channel www.rcsb.org/structure/8DE9

There are definitely automated solutions. Andreas Engel, Luca Rima and Thomas Braun have an interesting one that was described in www.pnas.org/doi/full/10.... from 2019 and probably better now. It uses a UV cleavable linker to elute from the beads and is all coupled into a robotic system

Can I be included please

How do we use these? It doesn’t work for me

Thank you!