Jakob Farnung
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Jakob Farnung
@jakobfarnung.bsky.social
340 followers 300 following 8 posts
PostDoc Schulman Lab, MPI Biochemistry; formerly Bode Lab, ETH Zürich
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jakobfarnung.bsky.social
Using a genome-wide CRISPR screen we identified the SCF-component FBXO31 as a selective reader of C-terminal amides. FBXO31 is a quality control factor, agnostically recognizing any amidated C-terminal amino acid. We propose that oxidative stress can cause spontaneous fragmentation of the peptide-backbone to leading to formation of C-terminally amidated proteins (CTAP).
January 29, 2025 at 4:29 PM
jakobfarnung.bsky.social
We combined chemical biology and genetic tools to devise a cellular screening platform to identify modified amino acid degrons (MAAD) that induce protein degradation. Surprisingly the smallest possible change from C-terminal carboxylic acid to an amide induces efficient degradation.
January 29, 2025 at 4:29 PM