Bluesky handles: @biorxivpreprint.bsky.social‬ ‪@perrimonlab.bsky.social‬ @addgene.bsky.social‬ ‪@dgrc.bsky.social‬ @bdsc.bsky.social‬ @damonrunyon.org‬ Collaborators: ‪@aliceyting.bsky.social‬, Steve Carr, John Asara.

Huge thanks to my amazing co-authors, mentors, and lab mates! Also grateful to the @DRSC_TRiP and fly community for resources and support 🪰, @DamonRunyon and @NIH for funding.
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Plasmids 🧬, cell lines 🧫, and fly lines 🪰are available NOW from @Addgene, @DrosDGRC, and Bloomington Drosophila Stock Center (BDSC)! A web-based portal to visualize our snRNA-seq data is at www.flyrnai.org/scRNA/body_l...
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Why does this matter?💡
Mapping the sources of circulating proteins unlocks new ways to study biology and disease. This atlas can help others explore tissues (e.g. brain), proteins (e.g. Lectins), and processes (e.g. inter-organ communication) - or even discover biomarkers.
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Amazingly, we also found tissue-specific secreted proteins that accumulate in entirely different tissues! These may be remote-control factors that allow two organs to communicate (aka inter-organ communication).
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We discovered a gold mine of 535 circulating proteins! Highlights:
-Tissue-specific secreted proteins
-Unexpected sources like glia 🧠
-Shed ectodomains of transmembrane proteins
-Uncharacterized proteins
-Human-conserved proteins 🧬
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We built an atlas of secreted proteins in Drosophila blood from 10 different tissues using complementary ‘omics tools 🧬- transgenic TurboID proximity labeling, mass spectrometry, single nucleus RNA-seq, and CRISPR knock-in flies.
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Extracellular proteins are critical for basic biology (development, physiology, immunity, etc), and thousands circulate in our blood plasma🩸. We know they’re secreted from cells in different tissues… so… *which* tissues secrete *which* proteins?🤔 🤷
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