International Society for Protein Termini (ISPT)
@ispt-proteinterm.bsky.social
Non-profit society of scientists devoted to understanding the molecular mechanisms and biological role of protein termini in plants, animals, fungi and bacteria. Conference every second year.
Website: https://proteintermini.org/
Website: https://proteintermini.org/
Pinned
See you at Palazzo dei Normanni in Palermo for the 2026 Protein Termini Conference | Co- and post-translational modifications | Proteolytic processing and degradation pathways | Microbes, plants, animals #ProteinTermini2026 #Proteostasis #ProteinModifications #PalazzoDeiNormanni #Palermo2026
Reposted by International Society for Protein Termini (ISPT)
Want to assess N-terminal acetyltransferases without radioactive labelling or mass spec? Look here! @ispt-proteinterm.bsky.social @cellsignal.com
October 27, 2025 at 8:25 PM
Want to assess N-terminal acetyltransferases without radioactive labelling or mass spec? Look here! @ispt-proteinterm.bsky.social @cellsignal.com
See you at Palazzo dei Normanni in Palermo for the 2026 Protein Termini Conference | Co- and post-translational modifications | Proteolytic processing and degradation pathways | Microbes, plants, animals #ProteinTermini2026 #Proteostasis #ProteinModifications #PalazzoDeiNormanni #Palermo2026
October 26, 2025 at 6:59 PM
See you at Palazzo dei Normanni in Palermo for the 2026 Protein Termini Conference | Co- and post-translational modifications | Proteolytic processing and degradation pathways | Microbes, plants, animals #ProteinTermini2026 #Proteostasis #ProteinModifications #PalazzoDeiNormanni #Palermo2026
Reposted by International Society for Protein Termini (ISPT)
We are advertising a PhD project investigating a cellular mechanism that ensures protein quality control during mRNA translation in plants. Please get in touch on here or via email if you are interested and I can provide further details!
Lab website: sites.google.com/site/danielg...
Lab website: sites.google.com/site/danielg...
Daniel Gibbs Lab
Welcome to the lab website of Professor Daniel Gibbs
@ University of Birmingham
sites.google.com
October 14, 2025 at 2:07 PM
We are advertising a PhD project investigating a cellular mechanism that ensures protein quality control during mRNA translation in plants. Please get in touch on here or via email if you are interested and I can provide further details!
Lab website: sites.google.com/site/danielg...
Lab website: sites.google.com/site/danielg...
Reposted by International Society for Protein Termini (ISPT)
Uptake of anti-cancer drugs depends on N-terminally acetylated anion channels | @ispt-proteinterm.bsky.social www.nature.com/articles/s42...
NAA60 facilitates LRRC8A- and LRRC8D-mediated platinum drug uptake - Communications Biology
Genome-wide CRISPR/Cas9 screening identifies the N-terminal acetyltransferase NAA60 to facilitate cis- and carboplatin drug sensitivity, most likely by neutralizing Nt acetylation of the LRRC8A/D VRAC subunits regulating substrate permeability.
www.nature.com
October 7, 2025 at 11:03 AM
Uptake of anti-cancer drugs depends on N-terminally acetylated anion channels | @ispt-proteinterm.bsky.social www.nature.com/articles/s42...
Reposted by International Society for Protein Termini (ISPT)
A Cell-Potent Bisubstrate Inhibitor to Probe NatD Acetyltransferase Activity | The inhibitor effectively reduced cellular Nα-acetylation on histone H4, leading to reduced migration in lung cancer cells | @acs.org ACS Chemical Biology @ispt-proteinterm.bsky.social pubs.acs.org/doi/10.1021/...
A Cell-Potent Bisubstrate Inhibitor to Probe NatD Acetyltransferase Activity
N-terminal acetyltransferase D (NatD) is a highly selective enzyme that acetylates the α-N-terminal amine of histones H4 and H2A, which share the SGRGK motif. Elevated NatD expression has been observe...
pubs.acs.org
October 5, 2025 at 12:07 PM
A Cell-Potent Bisubstrate Inhibitor to Probe NatD Acetyltransferase Activity | The inhibitor effectively reduced cellular Nα-acetylation on histone H4, leading to reduced migration in lung cancer cells | @acs.org ACS Chemical Biology @ispt-proteinterm.bsky.social pubs.acs.org/doi/10.1021/...
Protein N-terminal acetylation regulates the growth of rice plants | #NAA30 #NatC #rice #acetylation www.sciencedirect.com/science/arti...
September 25, 2025 at 9:45 AM
Protein N-terminal acetylation regulates the growth of rice plants | #NAA30 #NatC #rice #acetylation www.sciencedirect.com/science/arti...
Reposted by International Society for Protein Termini (ISPT)
Two new volumes of Methods in Enzymology with 29 chapters/papers on Protein termini methods and reviews are now out! Thx to all authors!🙌 @ispt-proteinterm.bsky.social #N-degron #prenylation #myristoylation #formylation #arginylation #oxidation #acetylation www.sciencedirect.com/bookseries/m...
Methods in Enzymology | Volume 719: Protein Termini - Part B | ScienceDirect.com by Elsevier
Read the latest chapters of Methods in Enzymology at ScienceDirect.com, Elsevier’s leading platform of peer-reviewed scholarly literature
www.sciencedirect.com
September 25, 2025 at 9:17 AM
Two new volumes of Methods in Enzymology with 29 chapters/papers on Protein termini methods and reviews are now out! Thx to all authors!🙌 @ispt-proteinterm.bsky.social #N-degron #prenylation #myristoylation #formylation #arginylation #oxidation #acetylation www.sciencedirect.com/bookseries/m...
Reposted by International Society for Protein Termini (ISPT)
Developing leaves form a spatiotemporal oxygen gradient that is sensed through the oxygen sensing machinery and directs morphogenesis | New preprint from @daanweits.bsky.social and @syno2xis.bsky.social labs #N-degron #Cysteine #hypoxia @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.1...
September 23, 2025 at 7:38 AM
Developing leaves form a spatiotemporal oxygen gradient that is sensed through the oxygen sensing machinery and directs morphogenesis | New preprint from @daanweits.bsky.social and @syno2xis.bsky.social labs #N-degron #Cysteine #hypoxia @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.1...
Reposted by International Society for Protein Termini (ISPT)
Anatomy and habitat shape the oxygen sensing machinery of flowering plants | new preprint from Licausi lab @syno2xis.bsky.social #hypoxia #N-degron @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.1...
Anatomy and habitat shape the oxygen sensing machinery of angiosperms
Hypoxia sensing via the Cys/Arg branch of the N-degron pathway (Cys-NDP) is central for flooding responses in plants, yet how evolutionary and ecological factors have shaped the core oxygen sensing me...
www.biorxiv.org
September 18, 2025 at 12:40 PM
Anatomy and habitat shape the oxygen sensing machinery of flowering plants | new preprint from Licausi lab @syno2xis.bsky.social #hypoxia #N-degron @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.1...
New review on the generation and consequences of N-terminal proteoform diversity from Evan Morrison and Olivia Rissland | #N-degron #RNA @cp-cellreports.bsky.social www.sciencedirect.com/science/arti...
The generation and consequences of N-terminal proteoform diversity
N-terminal proteoforms are protein variants with altered N termini that arise from RNA-driven processes, such as alternative promoter usage, splicing,…
www.sciencedirect.com
September 17, 2025 at 8:09 AM
New review on the generation and consequences of N-terminal proteoform diversity from Evan Morrison and Olivia Rissland | #N-degron #RNA @cp-cellreports.bsky.social www.sciencedirect.com/science/arti...
Targeted degradation of pathogenic TDP-43 proteins in amyotrophic lateral sclerosis using the AUTOTAC platform | Yong Tae Kwon lab | #N-degron www.biorxiv.org/content/10.1...
Targeted degradation of pathogenic TDP-43 proteins in amyotrophic lateral sclerosis using the AUTOTAC platform
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the progressive loss of motor neurons and the cytoplasmic aggregation of misfolded proteins in the spinal cord...
www.biorxiv.org
September 8, 2025 at 11:07 AM
Targeted degradation of pathogenic TDP-43 proteins in amyotrophic lateral sclerosis using the AUTOTAC platform | Yong Tae Kwon lab | #N-degron www.biorxiv.org/content/10.1...
A synthetic ERFVII-dependent circuit in yeast sheds light on the regulation of early hypoxic responses of plants | Preprint from @mikellavi.bsky.social @beagiuntoli.bsky.social @syno2xis.bsky.social #N-degron #hypoxia www.biorxiv.org/content/10.1...
A synthetic ERFVII-dependent circuit in yeast sheds light on the regulation of early hypoxic responses of plants
Plants face hypoxic conditions either chronically, as particular tissues are characterized by fluctuating or stable low oxygen levels, or acutely, when flooded. In vascular plants, transcriptional ada...
www.biorxiv.org
September 8, 2025 at 10:15 AM
A synthetic ERFVII-dependent circuit in yeast sheds light on the regulation of early hypoxic responses of plants | Preprint from @mikellavi.bsky.social @beagiuntoli.bsky.social @syno2xis.bsky.social #N-degron #hypoxia www.biorxiv.org/content/10.1...
Reposted by International Society for Protein Termini (ISPT)
Want to know how lipidation of some nascent chains takes place by NMT2-- Check our latest work on how NAC couples Protein Synthesis with Nascent Polypeptide Myristoylation on the Ribosome out today @embojournal.org: www.embopress.org/doi/full/10....
August 26, 2025 at 11:00 AM
Want to know how lipidation of some nascent chains takes place by NMT2-- Check our latest work on how NAC couples Protein Synthesis with Nascent Polypeptide Myristoylation on the Ribosome out today @embojournal.org: www.embopress.org/doi/full/10....
Reposted by International Society for Protein Termini (ISPT)
Nitric oxide promotes cysteine N-degron proteolysis through control of oxygen availability | Important finding by Kim, Tian, Ratcliffe and Keeley @ox.ac.uk @ispt-proteinterm.bsky.social #N-degron www.pnas.org/doi/10.1073/...
Nitric oxide promotes cysteine N-degron proteolysis through control of oxygen availability | PNAS
Selected proteins containing an N-terminal cysteine (Nt-Cys) are subjected to rapid,
O2-dependent proteolysis via the Cys/Arg-branch of the N-degro...
www.pnas.org
August 20, 2025 at 10:39 AM
Nitric oxide promotes cysteine N-degron proteolysis through control of oxygen availability | Important finding by Kim, Tian, Ratcliffe and Keeley @ox.ac.uk @ispt-proteinterm.bsky.social #N-degron www.pnas.org/doi/10.1073/...
Reposted by International Society for Protein Termini (ISPT)
Disease resistance in rice steered by a long non-coding RNA via protein N-terminal acetylation | @ispt-proteinterm.bsky.social advanced.onlinelibrary.wiley.com/doi/10.1002/...
Gene Editing of a Susceptibility LncRNA Enhances Broad‐Spectrum Disease Resistance in Rice without Developmental Trade‐Offs
Editing susceptibility lncRNAs offers a strategy to enhance disease resistance without yield loss. RESIS, a domestication-acquired, pathogen-induced lncRNA, promotes protein N-terminal acetylation th...
advanced.onlinelibrary.wiley.com
August 19, 2025 at 12:51 PM
Disease resistance in rice steered by a long non-coding RNA via protein N-terminal acetylation | @ispt-proteinterm.bsky.social advanced.onlinelibrary.wiley.com/doi/10.1002/...
Melon wrinkles linked to protein N-terminal acetylation. link.springer.com/article/10.1...
Fine mapping and prediction of a candidate gene for wrinkled rind in melon (Cucumis melo L.) - Theoretical and Applied Genetics
Key message The Cmwr locus was fine mapped to chromosome 2 in wrinkled-rind melon, and MELO3C010304.2 (CmNaa35, encoding an N-α-acetyltransferase) was identified as the most likely candidate gene for ...
link.springer.com
August 19, 2025 at 12:20 PM
Melon wrinkles linked to protein N-terminal acetylation. link.springer.com/article/10.1...
Reposted by International Society for Protein Termini (ISPT)
Global profiling of N-terminal cysteine–dependent degradation mechanisms | PNAS | @ispt-proteinterm.bsky.social www.pnas.org/doi/10.1073/...
Global profiling of N-terminal cysteine–dependent degradation mechanisms | PNAS
Hypoxia, a condition characterized by insufficient oxygen supply, challenges cellular
homeostasis and energy production, triggering adaptive respon...
www.pnas.org
August 18, 2025 at 1:09 PM
Global profiling of N-terminal cysteine–dependent degradation mechanisms | PNAS | @ispt-proteinterm.bsky.social www.pnas.org/doi/10.1073/...
Reposted by International Society for Protein Termini (ISPT)
Our 'Spotlight' discussing an excellent recent paper by @lauradc.bsky.social @n-end-rules.bsky.social & @syno2xis.bsky.social et al on the evolution of oxygen signalling in land plants is now online at Molecular Plant.
Spotlight: tinyurl.com/7ydn6p4a
Original paper: tinyurl.com/yw5yd4f8
Spotlight: tinyurl.com/7ydn6p4a
Original paper: tinyurl.com/yw5yd4f8
The evolutionary rise of oxygen sensing in land plants
Land plants evolved from aquatic green algal ancestors to dominate terrestrial ecosystems
approximately 480 million years ago during the Ordovician period (Hammarlund et al.,
2020). This major event i...
www.cell.com
August 7, 2025 at 2:48 PM
Our 'Spotlight' discussing an excellent recent paper by @lauradc.bsky.social @n-end-rules.bsky.social & @syno2xis.bsky.social et al on the evolution of oxygen signalling in land plants is now online at Molecular Plant.
Spotlight: tinyurl.com/7ydn6p4a
Original paper: tinyurl.com/yw5yd4f8
Spotlight: tinyurl.com/7ydn6p4a
Original paper: tinyurl.com/yw5yd4f8
Reposted by International Society for Protein Termini (ISPT)
📢 Our August issue is online!
🎨 The cover highlights a Feature Review from Stephanie Rosswag de Souza, @gzaffagnini.bsky.social, and @bokelab.bsky.social on how proteostasis functions in cellular dormancy across a number of examples, from yeast to adult quiescent stem cells and vertebrate oocytes.
🎨 The cover highlights a Feature Review from Stephanie Rosswag de Souza, @gzaffagnini.bsky.social, and @bokelab.bsky.social on how proteostasis functions in cellular dormancy across a number of examples, from yeast to adult quiescent stem cells and vertebrate oocytes.
August 7, 2025 at 2:53 PM
📢 Our August issue is online!
🎨 The cover highlights a Feature Review from Stephanie Rosswag de Souza, @gzaffagnini.bsky.social, and @bokelab.bsky.social on how proteostasis functions in cellular dormancy across a number of examples, from yeast to adult quiescent stem cells and vertebrate oocytes.
🎨 The cover highlights a Feature Review from Stephanie Rosswag de Souza, @gzaffagnini.bsky.social, and @bokelab.bsky.social on how proteostasis functions in cellular dormancy across a number of examples, from yeast to adult quiescent stem cells and vertebrate oocytes.
Zebrafish model shows that NAA15 and N-terminal acetylation defects cause heart disease | Independently confirms the causal link between pathogenic NAA15 variants and congenital heart disease | Burns lab @bostonchildrens.bsky.social www.biorxiv.org/content/10.1...
August 7, 2025 at 12:25 PM
Zebrafish model shows that NAA15 and N-terminal acetylation defects cause heart disease | Independently confirms the causal link between pathogenic NAA15 variants and congenital heart disease | Burns lab @bostonchildrens.bsky.social www.biorxiv.org/content/10.1...
Reposted by International Society for Protein Termini (ISPT)
Mechanism for voltage-gated channel inactivation uncovered | N-terminal acetylation of the intracellular domain of the Shaker potassium channel is key for plugging the pore | By Kenton Swartz lab in @nature.com @ispt-proteinterm.bsky.social www.nature.com/articles/s41...
August 7, 2025 at 8:11 AM
Mechanism for voltage-gated channel inactivation uncovered | N-terminal acetylation of the intracellular domain of the Shaker potassium channel is key for plugging the pore | By Kenton Swartz lab in @nature.com @ispt-proteinterm.bsky.social www.nature.com/articles/s41...
Reposted by International Society for Protein Termini (ISPT)
How to define degrons and E3 ligase specificities by peptide pulldowns | Methods in Enzymology | Kwon and Ji labs | #KCMF1 #Cysteine #Arg/N-degron @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
August 7, 2025 at 7:11 AM
How to define degrons and E3 ligase specificities by peptide pulldowns | Methods in Enzymology | Kwon and Ji labs | #KCMF1 #Cysteine #Arg/N-degron @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
Reposted by International Society for Protein Termini (ISPT)
Using a specific antibody to detect proteins carrying N-terminal formylation | Methods in Enzymology | Hwang lab @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
August 7, 2025 at 6:54 AM
Using a specific antibody to detect proteins carrying N-terminal formylation | Methods in Enzymology | Hwang lab @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
Reposted by International Society for Protein Termini (ISPT)
Assessing protein N-terminal myristoylation. Detailed protocols by Ed Tate's group in Methods in Enzymology. #lipidation @ispt-proteinterm.bsky.social @imperialcollegeldn.bsky.social www.sciencedirect.com/science/arti...
August 6, 2025 at 7:53 AM
Assessing protein N-terminal myristoylation. Detailed protocols by Ed Tate's group in Methods in Enzymology. #lipidation @ispt-proteinterm.bsky.social @imperialcollegeldn.bsky.social www.sciencedirect.com/science/arti...
Reposted by International Society for Protein Termini (ISPT)
Can we modulate plant oxygen sensing to enhance flood resilience?🌿🔧
www.biorxiv.org/content/10.1...
This is one of two preprints out this week from the @syno2xis.bsky.social and @emilyflashman.bsky.social labs, two angles on how PCOs shape plant flood resilience. Don’t miss the full story!
www.biorxiv.org/content/10.1...
This is one of two preprints out this week from the @syno2xis.bsky.social and @emilyflashman.bsky.social labs, two angles on how PCOs shape plant flood resilience. Don’t miss the full story!
Manipulating plant oxygen sensing through NCO substitution reveals trade-offs between growth and flooding tolerance
The oxygen-dependent degradation of Ethylene Response Factors VII (ERFVIIs) through the N-degron pathway is central to regulating the transcriptional responses to hypoxia in vascular plants. Plant Cys...
www.biorxiv.org
July 28, 2025 at 2:16 PM
Can we modulate plant oxygen sensing to enhance flood resilience?🌿🔧
www.biorxiv.org/content/10.1...
This is one of two preprints out this week from the @syno2xis.bsky.social and @emilyflashman.bsky.social labs, two angles on how PCOs shape plant flood resilience. Don’t miss the full story!
www.biorxiv.org/content/10.1...
This is one of two preprints out this week from the @syno2xis.bsky.social and @emilyflashman.bsky.social labs, two angles on how PCOs shape plant flood resilience. Don’t miss the full story!