idanfrumkin.bsky.social
@idanfrumkin.bsky.social
December 25, 2023 at 11:07 AM
5/5
Finally, we show that random proteins can improve through beneficial mutations, enhancing RamF's efficiency as a MazF inhibitor. In summary, our work provides a mechanistic basis for how de novo gene birth produces functional proteins benefiting cells under stress.
www.nature.com/articles/s41...
Finally, we show that random proteins can improve through beneficial mutations, enhancing RamF's efficiency as a MazF inhibitor. In summary, our work provides a mechanistic basis for how de novo gene birth produces functional proteins benefiting cells under stress.
www.nature.com/articles/s41...
December 25, 2023 at 11:06 AM
5/5
Finally, we show that random proteins can improve through beneficial mutations, enhancing RamF's efficiency as a MazF inhibitor. In summary, our work provides a mechanistic basis for how de novo gene birth produces functional proteins benefiting cells under stress.
www.nature.com/articles/s41...
Finally, we show that random proteins can improve through beneficial mutations, enhancing RamF's efficiency as a MazF inhibitor. In summary, our work provides a mechanistic basis for how de novo gene birth produces functional proteins benefiting cells under stress.
www.nature.com/articles/s41...
4/5
If RamF inhibits MazF indirectly, why doesn't it inhibit other MazF homologs? We found that N’ terminus tagging of MazF prevents its degradation and inhibition by RamF, suggesting the N’ of MazF is critical for toxin degradation. A chimeric toxin with MazF’s N’ terminus was inhibited by RamF.
If RamF inhibits MazF indirectly, why doesn't it inhibit other MazF homologs? We found that N’ terminus tagging of MazF prevents its degradation and inhibition by RamF, suggesting the N’ of MazF is critical for toxin degradation. A chimeric toxin with MazF’s N’ terminus was inhibited by RamF.
December 25, 2023 at 11:04 AM
4/5
If RamF inhibits MazF indirectly, why doesn't it inhibit other MazF homologs? We found that N’ terminus tagging of MazF prevents its degradation and inhibition by RamF, suggesting the N’ of MazF is critical for toxin degradation. A chimeric toxin with MazF’s N’ terminus was inhibited by RamF.
If RamF inhibits MazF indirectly, why doesn't it inhibit other MazF homologs? We found that N’ terminus tagging of MazF prevents its degradation and inhibition by RamF, suggesting the N’ of MazF is critical for toxin degradation. A chimeric toxin with MazF’s N’ terminus was inhibited by RamF.
3/5
Through genetic and molecular experiments, we demonstrated that RamF modulated protein homeostasis by interacting with chaperones, leading to MazF proteolysis and loss of toxicity. Interestingly, RamF-producing cells experienced no major transcriptional changes or significant growth defects.
Through genetic and molecular experiments, we demonstrated that RamF modulated protein homeostasis by interacting with chaperones, leading to MazF proteolysis and loss of toxicity. Interestingly, RamF-producing cells experienced no major transcriptional changes or significant growth defects.
December 25, 2023 at 11:02 AM
3/5
Through genetic and molecular experiments, we demonstrated that RamF modulated protein homeostasis by interacting with chaperones, leading to MazF proteolysis and loss of toxicity. Interestingly, RamF-producing cells experienced no major transcriptional changes or significant growth defects.
Through genetic and molecular experiments, we demonstrated that RamF modulated protein homeostasis by interacting with chaperones, leading to MazF proteolysis and loss of toxicity. Interestingly, RamF-producing cells experienced no major transcriptional changes or significant growth defects.
2/5
We identified ~2,000 random genes promoting growth, able to inhibit a variety of toxins by reducing transcription from the promoter driving toxin expression. Additionally, one random protein, named Random antitoxin of MazF (RamF), inhibited MazF specifically in a promoter-independent manner.
We identified ~2,000 random genes promoting growth, able to inhibit a variety of toxins by reducing transcription from the promoter driving toxin expression. Additionally, one random protein, named Random antitoxin of MazF (RamF), inhibited MazF specifically in a promoter-independent manner.
December 25, 2023 at 11:00 AM
2/5
We identified ~2,000 random genes promoting growth, able to inhibit a variety of toxins by reducing transcription from the promoter driving toxin expression. Additionally, one random protein, named Random antitoxin of MazF (RamF), inhibited MazF specifically in a promoter-independent manner.
We identified ~2,000 random genes promoting growth, able to inhibit a variety of toxins by reducing transcription from the promoter driving toxin expression. Additionally, one random protein, named Random antitoxin of MazF (RamF), inhibited MazF specifically in a promoter-independent manner.