thanks for sharing this

Ah, I see your problem. You think the fancy search engine is an actual person.

yes, thanks for the discussion!

this is also correctly predicted

yep correct, the small protein is the green box in bottom right. This is a large membrane protein. The interacting region of the first protein can be verified by homology modelling. All the high confidence regions predicted by AF3 can be verified by homology, and inter domain packing is also good.

PAE looks like this. The iPTM score is also 0.74, so these helices are really unreliable, and they take up a considerable amount of amino acids in the total sequence.

ah I see, thanks

thanks, yeah I figured because manual inspection of the sequence and IDR prediction server also spit out similar disordered behaviour for these regions. Most of these are just poly-Ns, some need to be checked more carefully.

ohh that low confidence in the middle of the curl..yikes.

ohh do you have a visual example of 'curled' models?

thanks!

🙌🙌

haha it is art as long as it provokes a human emotion, pain in this case

yep, it was apparently a fabrication issue because we didn't notice anything strange while handling and preparing this grid, it was only clear at the screening stage

ABC transporters contributing to drug resistance in bacteria: doi.org/10.1016/S001...

trying to fix the student numbers right here 😬 hopefully my friends pop in too