6-way multiplexing at the first step allows 10-20 libraries to be made in parallel

Great work Neesha and thanks to our collaborators and our funders

TrAEL-seq read density reflects the speed or replication forks, allowing quantification of interactions with genomic elements such as transcription

Great work lead by Hanane Hadj-Moussa and Megan Ulusan

Babraham Institute @babrahaminst.bsky.social

#BBSRC @ukri.org

AMPK orchestrates responses to low nutrient availability from yeast to humans…

…including regulation of fatty acid synthesis through phosphorylation of the same enzyme

This highly conserved regulatory system presents a promising and unexplored target for healthy ageing research across Eukaryotes

Controlling Acetyl coenzyme A availability underlies all these effects

Acetyl coenzyme A is the building block for fatty acids but excess acetyl coenzyme A availability is detrimental

A2A cells make the fatty acid they need but get rid of excess acetyl coenzyme A into mitochondria

but AMPK activation turns off fatty acid production, which is a problem

Our A2A mutant combines AMPK activation with a mutation that allows continued fatty acid synthesis

The majority of A2A cells maintain fitness very late in life even though they don't live any longer

AMPK activity is widely considered to be beneficial for ageing health

We show that AMPK activation in budding yeast allows some cells to age without senescence on a normal diet...