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HOLTRA
@holtra-tech.bsky.social
Technology for the future of nanoparticle analysis
https://www.holtra.tech
Based in Gothenburg, Sweden

Here for all things nano, extracellular vesicle, #nanomedicine, #virus, #exosome, #EV, #LNP
DAISY nanoparticle tracking analysis is a powerful method for refractive index determination of nanoparticles!
learn more here: www.holtra.tech
August 20, 2025 at 2:45 PM
Or between extracellular vesicles and lipoproteins!
August 20, 2025 at 2:45 PM
This means that you can easily distinguish between protein aggregates and non-protein contaminants..
August 20, 2025 at 2:45 PM
Put simply, refractive index measurements allow you to differentiate between populations of particles within your sample more effectively
August 20, 2025 at 2:45 PM
In summary, holographic microscopy offers label free, single shot, high resolution, high frame rate, 3D imaging! It sounds almost too good to be true, and that is why we are so convinced of it’s potential and excited to be developing microscopes around this technology!
August 12, 2025 at 9:00 AM
For holographic microscopy, instead of physically illuminating the interference patterns to reveal the hologram, the hologram is reconstructed computationally. With rapid advances in processing power for these kinds of tasks, the potential of holographic microscopy is only growing!
August 12, 2025 at 8:53 AM
This means that from a single light exposure a hologram captures a 3D representation of the object! The images of the mouse below are produced by viewing the same hologram from different angles!
August 12, 2025 at 8:53 AM
To create a hologram, the light coming from the object is merged with another beam, creating interference pattern between the two beams. When the object is removed and the interference pattern illuminated, light is diffracted and a 3D image of the original object appears.
August 12, 2025 at 8:53 AM
When light from an illuminated object is focussed and captured by a camera a 2D image is recorded. A hologram however is a 3D representation of the object of interest and is recorded differently. Relying on interference patterns similar to those seen between waves on water.
August 12, 2025 at 8:53 AM
Firstly, what is a hologram?
holograms, like the Visa dove below, are very familiar. but what are they? Much like a recording of a sound, e.g. music, a hologram is a 3D “recording” of an illuminated object that can be replayed, even once the object is removed. But how are they created?
August 12, 2025 at 8:53 AM
For this study DAISY was used to monitor the purity and consistency of cell culture-derived exosome preparations. Check out our application not on DAISY for extracellular vesicles to learn more.
www.holtra.tech/en/applicati...
Extracellular Vesicles
The future of particle characterization. Holographic particle tracking.
www.holtra.tech
August 11, 2025 at 9:23 AM
Get in touch with us if you want to see for yourself how DAISY can revolutionise your nanoparticle analysis!
www.holtra.tech
Holographic particle tracking analysis instrument
Analytical instruments and services for colloidal particles in liquids - providing unprecedentedly rich and precise information
www.holtra.tech
August 8, 2025 at 8:23 AM
To measure phase shift, DAISY employs digital holographic microscopy (DHM). DHM separates phase and intensity information to generate a 3D image of the light scattered by each particle from a single exposure.
August 8, 2025 at 8:23 AM
Alongside size, DAISY measures the refractive index of each particle. It does this by directly measuring the phase shift of light passing through the particles. Denser materials give higher phase shift which means that, uniquely, DAISY can differentiate particles based on their composition!
August 8, 2025 at 8:23 AM
Nanoparticle tracking analysis (NTA) records particles diffusing in solution. The smaller the particle the faster the diffusion. This is how NTA assigns a size.
August 8, 2025 at 8:23 AM