DAISY nanoparticle tracking analysis is a powerful method for refractive index determination of nanoparticles!
learn more here: www.holtra.tech

Or between extracellular vesicles and lipoproteins!

This means that you can easily distinguish between protein aggregates and non-protein contaminants..

Put simply, refractive index measurements allow you to differentiate between populations of particles within your sample more effectively

In summary, holographic microscopy offers label free, single shot, high resolution, high frame rate, 3D imaging! It sounds almost too good to be true, and that is why we are so convinced of it’s potential and excited to be developing microscopes around this technology!

For holographic microscopy, instead of physically illuminating the interference patterns to reveal the hologram, the hologram is reconstructed computationally. With rapid advances in processing power for these kinds of tasks, the potential of holographic microscopy is only growing!

This means that from a single light exposure a hologram captures a 3D representation of the object! The images of the mouse below are produced by viewing the same hologram from different angles!

To create a hologram, the light coming from the object is merged with another beam, creating interference pattern between the two beams. When the object is removed and the interference pattern illuminated, light is diffracted and a 3D image of the original object appears.

When light from an illuminated object is focussed and captured by a camera a 2D image is recorded. A hologram however is a 3D representation of the object of interest and is recorded differently. Relying on interference patterns similar to those seen between waves on water.

Firstly, what is a hologram?
holograms, like the Visa dove below, are very familiar. but what are they? Much like a recording of a sound, e.g. music, a hologram is a 3D “recording” of an illuminated object that can be replayed, even once the object is removed. But how are they created?

For this study DAISY was used to monitor the purity and consistency of cell culture-derived exosome preparations. Check out our application not on DAISY for extracellular vesicles to learn more.
www.holtra.tech/en/applicati...

Get in touch with us if you want to see for yourself how DAISY can revolutionise your nanoparticle analysis!
www.holtra.tech

To measure phase shift, DAISY employs digital holographic microscopy (DHM). DHM separates phase and intensity information to generate a 3D image of the light scattered by each particle from a single exposure.

Alongside size, DAISY measures the refractive index of each particle. It does this by directly measuring the phase shift of light passing through the particles. Denser materials give higher phase shift which means that, uniquely, DAISY can differentiate particles based on their composition!

Nanoparticle tracking analysis (NTA) records particles diffusing in solution. The smaller the particle the faster the diffusion. This is how NTA assigns a size.