DAISY nanoparticle tracking analysis is a powerful method for refractive index determination of nanoparticles!
learn more here: www.holtra.tech

Or between extracellular vesicles and lipoproteins!

This means that you can easily distinguish between protein aggregates and non-protein contaminants..

Put simply, refractive index measurements allow you to differentiate between populations of particles within your sample more effectively

This means that from a single light exposure a hologram captures a 3D representation of the object! The images of the mouse below are produced by viewing the same hologram from different angles!

To create a hologram, the light coming from the object is merged with another beam, creating interference pattern between the two beams. When the object is removed and the interference pattern illuminated, light is diffracted and a 3D image of the original object appears.

When light from an illuminated object is focussed and captured by a camera a 2D image is recorded. A hologram however is a 3D representation of the object of interest and is recorded differently. Relying on interference patterns similar to those seen between waves on water.

Firstly, what is a hologram?
holograms, like the Visa dove below, are very familiar. but what are they? Much like a recording of a sound, e.g. music, a hologram is a 3D “recording” of an illuminated object that can be replayed, even once the object is removed. But how are they created?

What is holographic microscopy?
And why are we so excited about it’s scientific potential?

Size is a key descriptor of extracellular vesicles #EV. In samples containing contaminating particles with an overlapping size distribution, size alone is insufficient. Measuring refractive index at the individual particle level enables differentiation of extracellular vesicles and lipoproteins.

To measure phase shift, DAISY employs digital holographic microscopy (DHM). DHM separates phase and intensity information to generate a 3D image of the light scattered by each particle from a single exposure.

Alongside size, DAISY measures the refractive index of each particle. It does this by directly measuring the phase shift of light passing through the particles. Denser materials give higher phase shift which means that, uniquely, DAISY can differentiate particles based on their composition!

Nanoparticle tracking analysis (NTA) records particles diffusing in solution. The smaller the particle the faster the diffusion. This is how NTA assigns a size.

What is DAISY?
DAISY is next generation nanoparticle tracking analysis!

DAISY nanoparticle tracking in action!
sample is 415 nm silica beads