#compneuro

This is my first 1st author paper and am very proud of it.
Thanks to my co-authors Anzal KS and my PhD mentor Prof. Upinder S. Bhalla, NCBS, Bangalore.
Feedback appreciated.
Link to the preprint:
www.biorxiv.org/content/10.1...
@biorxiv-neursci.bsky.social @biorxivpreprint.bsky.social

Even after 70 years of Hodgkin-Huxley models, gaps remain in our understanding of neural dynamics. Developing accurate neuronal models could bridge these gaps and provide deeper insights into the emergent behavior of biological neural networks in both health and disease. 11/n

In addition to elucidating the mechanisms underlying DBLO, which will result in more accurate and reliable models, we also provide a novel way to characterize a neuron’s morphological and electrotonic characteristics via its wide-spectrum impedance amplitude profile. 10/n

Combining these insights leads to models with experimentally high DBLO 9/n

Trivial mechanisms such as passive charging of neurons, spikes relayed from the axon initial segment, or high reversal potential of the principal potassium channel do not hold. 8/n

Dendrites contribute a depolarizing current during the repolarization phase of an action potential that raises the DBLO. Rapid potassium channel kinetics and appropriate sodium channel kinetics also result in high DBLO. 7/n

We found that many studies were ignoring liquid junction potential (LJP) correction, in the absence of which the sodium channels do not recover from inactivation after a single spike. 6/n

DBLO interacts with various other features such as calcium influx and bistable firing. Incorrect DBLO in models thus affects their realism and predictive power. 5/n

The mechanism behind this high DBLO are poorly understood. Many published models of CA1 pyramidal neurons do not match experimental DBLO. 4/n

Many neurons especially pyramidal neurons do not show this prominent after-hyperpolarization and instead show a high depolarization baseline offset (DBLO): 3/n

We are accustomed to seeing the following image of an action potential with a very strong after-hyperpolarization thanks to the seminal work by Hodgkin and Huxley: 2/n