Henri Niskanen
@henriniskanen.bsky.social
Postdoc @ Hnisz lab | Max Planck Institute for Molecule Genetics, Berlin | Condensates & Gene regulation | Past: PhD (Molecular Medicine, Uni. East Finland) MSc (biochemistry, Uni. Turku)
7/7
In summary, the killswitch is a versatile tool that alters condensate material properties in live cells. It revealed that condensate composition and functions rely on their microenvironments, paving the way for new biology and therapeutic strategies
In summary, the killswitch is a versatile tool that alters condensate material properties in live cells. It revealed that condensate composition and functions rely on their microenvironments, paving the way for new biology and therapeutic strategies
June 4, 2025 at 3:29 PM
7/7
In summary, the killswitch is a versatile tool that alters condensate material properties in live cells. It revealed that condensate composition and functions rely on their microenvironments, paving the way for new biology and therapeutic strategies
In summary, the killswitch is a versatile tool that alters condensate material properties in live cells. It revealed that condensate composition and functions rely on their microenvironments, paving the way for new biology and therapeutic strategies
3/7
To analyze compositional changes, we developed NuFANCI, a FACS-based method to isolate nuclear condensates. Applied to killswitch-targeted nucleoli, mass spectrometry revealed selective depletion of ~20 RNA-binding proteins, demonstrating condensate-material-property–dependent partitioning.
To analyze compositional changes, we developed NuFANCI, a FACS-based method to isolate nuclear condensates. Applied to killswitch-targeted nucleoli, mass spectrometry revealed selective depletion of ~20 RNA-binding proteins, demonstrating condensate-material-property–dependent partitioning.
June 4, 2025 at 3:29 PM
3/7
To analyze compositional changes, we developed NuFANCI, a FACS-based method to isolate nuclear condensates. Applied to killswitch-targeted nucleoli, mass spectrometry revealed selective depletion of ~20 RNA-binding proteins, demonstrating condensate-material-property–dependent partitioning.
To analyze compositional changes, we developed NuFANCI, a FACS-based method to isolate nuclear condensates. Applied to killswitch-targeted nucleoli, mass spectrometry revealed selective depletion of ~20 RNA-binding proteins, demonstrating condensate-material-property–dependent partitioning.
2/7
Using the nanobody system, killswitch can be targeted to a wide range of endogenous condensates, including nucleoli, nuclear speckles, chromocenters, and disease-specific condensates.
Using the nanobody system, killswitch can be targeted to a wide range of endogenous condensates, including nucleoli, nuclear speckles, chromocenters, and disease-specific condensates.
June 4, 2025 at 3:29 PM
2/7
Using the nanobody system, killswitch can be targeted to a wide range of endogenous condensates, including nucleoli, nuclear speckles, chromocenters, and disease-specific condensates.
Using the nanobody system, killswitch can be targeted to a wide range of endogenous condensates, including nucleoli, nuclear speckles, chromocenters, and disease-specific condensates.
1/7
Killswitch (KS) is a non-natural, self-associating micropeptide that can be genetically fused to condensate proteins or recruited via GFP-nanobody. It immobilizes scaffold proteins without affecting soluble pools—letting us perturb condensates selectively in live cells.
Killswitch (KS) is a non-natural, self-associating micropeptide that can be genetically fused to condensate proteins or recruited via GFP-nanobody. It immobilizes scaffold proteins without affecting soluble pools—letting us perturb condensates selectively in live cells.
June 4, 2025 at 3:29 PM
1/7
Killswitch (KS) is a non-natural, self-associating micropeptide that can be genetically fused to condensate proteins or recruited via GFP-nanobody. It immobilizes scaffold proteins without affecting soluble pools—letting us perturb condensates selectively in live cells.
Killswitch (KS) is a non-natural, self-associating micropeptide that can be genetically fused to condensate proteins or recruited via GFP-nanobody. It immobilizes scaffold proteins without affecting soluble pools—letting us perturb condensates selectively in live cells.
In summary, the killswitch is a versatile tool that alters condensate material properties in live cells. It revealed that condensate composition and functions rely on their microenvironments, paving the way for new biological insights and therapeutic strategies.
June 4, 2025 at 3:23 PM
In summary, the killswitch is a versatile tool that alters condensate material properties in live cells. It revealed that condensate composition and functions rely on their microenvironments, paving the way for new biological insights and therapeutic strategies.
3/7
To analyze compositional changes, we developed NuFANCI, a FACS-based method to isolate nuclear condensates. Applied to killswitch-targeted nucleoli, mass spectrometry revealed selective depletion of ~20 RNA-binding proteins, demonstrating condensate-material-property–dependent partitioning.
To analyze compositional changes, we developed NuFANCI, a FACS-based method to isolate nuclear condensates. Applied to killswitch-targeted nucleoli, mass spectrometry revealed selective depletion of ~20 RNA-binding proteins, demonstrating condensate-material-property–dependent partitioning.
June 4, 2025 at 3:21 PM
3/7
To analyze compositional changes, we developed NuFANCI, a FACS-based method to isolate nuclear condensates. Applied to killswitch-targeted nucleoli, mass spectrometry revealed selective depletion of ~20 RNA-binding proteins, demonstrating condensate-material-property–dependent partitioning.
To analyze compositional changes, we developed NuFANCI, a FACS-based method to isolate nuclear condensates. Applied to killswitch-targeted nucleoli, mass spectrometry revealed selective depletion of ~20 RNA-binding proteins, demonstrating condensate-material-property–dependent partitioning.
2/7
Using the nanobody system, killswitch can be targeted to a wide range of endogenous condensates, including nucleoli, nuclear speckles, chromocenters, and disease-specific condensates.
Using the nanobody system, killswitch can be targeted to a wide range of endogenous condensates, including nucleoli, nuclear speckles, chromocenters, and disease-specific condensates.
June 4, 2025 at 3:21 PM
2/7
Using the nanobody system, killswitch can be targeted to a wide range of endogenous condensates, including nucleoli, nuclear speckles, chromocenters, and disease-specific condensates.
Using the nanobody system, killswitch can be targeted to a wide range of endogenous condensates, including nucleoli, nuclear speckles, chromocenters, and disease-specific condensates.
Killswitch (KS) is a non-natural, self-associating micropeptide that can be genetically fused to condensate proteins or recruited via GFP-nanobody. It immobilizes scaffold proteins without affecting soluble pools—letting us perturb condensates selectively in live cells.
June 4, 2025 at 3:21 PM
Killswitch (KS) is a non-natural, self-associating micropeptide that can be genetically fused to condensate proteins or recruited via GFP-nanobody. It immobilizes scaffold proteins without affecting soluble pools—letting us perturb condensates selectively in live cells.