These work for immuno-EM IME, but for fluorescence might be different (or require a background reduction kit/protocol).

A short heated citrate buffer incubation can be used for antigen retrieval but keep it less than 15 minutes. Sodium borohydride (0.1%) incubation can also help with antigen retrieval. Happy to chat more!

If you can fix with acrolein over glut, that's going to be better for staining. An alternative is to do the staining prior following PFA fixation, fix with glut and then process for EM.

The Aves chicken one works well (Aves Lab, GFP‐1020) and I've also had success with an Invitrogen rabbit polyclonal (A11122).

If you were looking to avoid a specific host?

What species? Are you doing immuno-EM (and immuno gold or DAB) or IHC (DAB or fluorescence)? I know a few good GFP ones depending (I'll follow up with access to my computer). There's some tricks to help restore antigenicity with glut fixed tissues but if you can fix with acrolein it's better.

Once you start thinking of AI as a war on humanity, on human thought, on human inquiry, on human labor, on nuance and critical thinking, it slots in pretty seamlessly with the right wing ideological project, oligarchical political projects, big tech's political projects, etc

Remember how we always talk about there being many, many ways to resist? This may seem like a small one, but it’s actually HUGE.

This administration is absolutely hell bent on the eugenics of the disabled and chronically ill.

Wearing a health mask is an ENORMOUS act of resistance.

I miss Roxy Cerebellum

Thanks for involving us in this amazing work!! Very blessed to be involved and excited to see this out!! Congrats everyone 🎉

I forgot about tuition. In 🇨🇦 at least, student has to pay international rate tuition for grad school, which is often very $$. The amount may change (lower) in the second+ year of MSc and post-candidacy PhD, but this is very program and university dependent.

I feel like I was maybe in grad school for a year longer than if I had transferred from my MSc to PhD, but my funding was good. (And because in 🇨🇦 many grad stipends aren't taxed, I was technically making more as a grad student than postdoc). There's pros/cons to each way and it's very individualized

I fully completed my MSc and then a PhD. For me, that was really great, as it helped me build my CV to be more competitive for awards. Depending on country/program, unlike the USA, at least in Canada, thesis based MSc usually have a stipend. I also had access to USA student loans if needed.

A lot of places will encourage an MSc or have a direct entry (with different requirements) or encourage a transfer after a year from MSc to PhD (there's usually a transfer 'exam' in addition to quals/CE). As an early participant of the 🇺🇸➡️🇨🇦 for grad school pipeline, I'm happy to chat with anyone.

Thank you for including us!! Excited to have played a small part in this very cool study 🤩!!

Yesssssss 🎉🎉🎉🎉🎉