Michalis Gounis
@gounismichalis.bsky.social
Postdoc @Ivaskalab.bsky.social @BioscienceTurku (previously @CRUK-SI.bsky.social) with an interest in tumour microenvironment, metabolism and invasion #tumourmicroenviroment#metastasis
7/ Interestingly, disruption of Rab27s opposes EV release from primary tumour cells, but not from micrometastatic cells. Lastly, we show that the switch to nSM2-dependent EV release enables micrometastatic cells to influence #ECM deposition by fibroblasts to foster an invasive #microenvironment.
June 9, 2025 at 8:26 PM
7/ Interestingly, disruption of Rab27s opposes EV release from primary tumour cells, but not from micrometastatic cells. Lastly, we show that the switch to nSM2-dependent EV release enables micrometastatic cells to influence #ECM deposition by fibroblasts to foster an invasive #microenvironment.
4/ Given the importance of #EVs in metastatic niche priming, we sought to examine the relationship of EV release from micrometastatic cells to their altered metabolism. We found that decreased ability to synthesise glutathione is associated with increased EV release.
June 9, 2025 at 8:26 PM
4/ Given the importance of #EVs in metastatic niche priming, we sought to examine the relationship of EV release from micrometastatic cells to their altered metabolism. We found that decreased ability to synthesise glutathione is associated with increased EV release.
3/We further demonstrate that proline and glutathione pathways compete for glutamine-derived carbons in micrometastatic cells. The adoption of this metabolic profile, in which proline and glutathione levels are, respectively, elevated and suppressed, is a feature unique to micrometastatic cells.
June 9, 2025 at 8:26 PM
3/We further demonstrate that proline and glutathione pathways compete for glutamine-derived carbons in micrometastatic cells. The adoption of this metabolic profile, in which proline and glutathione levels are, respectively, elevated and suppressed, is a feature unique to micrometastatic cells.
2/ We generated isogenic cells from primary mammary tumours and their corresponding lung micrometastases. We show that micrometastatic cells increase proline production and circulating proline is significantly elevated in mice and patients with metastatic disease.
June 9, 2025 at 8:26 PM
2/ We generated isogenic cells from primary mammary tumours and their corresponding lung micrometastases. We show that micrometastatic cells increase proline production and circulating proline is significantly elevated in mice and patients with metastatic disease.
7/ Interestingly, disruption of Rab27s opposes EV release from primary tumour cells, but not from micrometastatic cells. Lastly, we show that the switch to nSM2-dependent EV release enables micrometastatic cells to influence #ECM deposition by fibroblasts to foster an invasive #microenvironment.
June 9, 2025 at 8:25 PM
7/ Interestingly, disruption of Rab27s opposes EV release from primary tumour cells, but not from micrometastatic cells. Lastly, we show that the switch to nSM2-dependent EV release enables micrometastatic cells to influence #ECM deposition by fibroblasts to foster an invasive #microenvironment.
4/ Given the importance of #EVs in metastatic niche priming, we sought to examine the relationship of EV release from micrometastatic cells to their altered metabolism. We found that decreased ability to synthesise glutathione is associated with increased EV release.
June 9, 2025 at 8:25 PM
4/ Given the importance of #EVs in metastatic niche priming, we sought to examine the relationship of EV release from micrometastatic cells to their altered metabolism. We found that decreased ability to synthesise glutathione is associated with increased EV release.
3/We further demonstrate that proline and glutathione pathways compete for glutamine-derived carbons in micrometastatic cells. The adoption of this metabolic profile, in which proline and glutathione levels are, respectively, elevated and suppressed, is a feature unique to micrometastatic cells.
June 9, 2025 at 8:25 PM
3/We further demonstrate that proline and glutathione pathways compete for glutamine-derived carbons in micrometastatic cells. The adoption of this metabolic profile, in which proline and glutathione levels are, respectively, elevated and suppressed, is a feature unique to micrometastatic cells.
2/ We generated isogenic cells from primary mammary tumours and their corresponding lung micrometastases. We show that micrometastatic cells increase proline production and circulating proline is significantly elevated in mice and patients with metastatic disease.
June 9, 2025 at 8:25 PM
2/ We generated isogenic cells from primary mammary tumours and their corresponding lung micrometastases. We show that micrometastatic cells increase proline production and circulating proline is significantly elevated in mice and patients with metastatic disease.