Thanks Filipe! I used 1-10 uL/min which correlates to what you would approximately find in your vasculature and urinary tract. My first paper has those citations, happy to send it to you!

I could not have done this work without the help of my mentor Joseph Sanfilippo, our collaborators Zil and Matthias, and the valuable feedback from the members of the lab (@anu-sharma.bsky.social, @ashuppara.bsky.social, @piyushsharma.bsky.social, and Jessica Palalay). (5/5)

Finally, we find that in flow cells can grow on glucose concentrations 1,000 time lower than in batch culture. These concentrations align with the affinity of bacterial glucose transporters, suggesting that bacteria have evolved to scavenge low concentrations of nutrients to support growth. (4/5)

In contrast, our microfluidic experiments revealed that cells can grow on extremely low levels of carbon and nitrogen when delivered by flow. (3/5)

In batch, we found that cells require 0.01% glucose and 0.1 mM ammonium chloride for growth. (2/5)

No but now I definitely have to! It very much feels like that was the approach. Especially when the acknowledgments noted a few subject matter experts and none of them were biologists.

Thanks so much!

Hi Souvik, can I get added to this! Thanks.