Happy to host them. We have a paid summer internship program here at @tgenresearch.bsky.social

Read the original work here academic.oup.com/neuro-oncolo...

Why does the telomere interact with these distant repeats? We speculate that this is key for maintaining nuclear organization or chromosome territories and may involve nuclear orphan receptors. Many questions remain. Read the paper to dive into the data 👇 #TelomereC #Genomics #Chromatin #Epigenetics

We validated these contacts with classic 3C/PCR and confirmed the 3D proximity using Southern Blotting and stunning 3D FISH data, proving these are real, higher-order chromatin structures.

Specifically, we found strong enrichment at three classes of repetitive elements: Interstitial Telomeric Sequences (ITS), Telomere-Associated Repeat 1 (TAR1), and D20S16 elements.

The telomeric interactome spans the entire genome! While contacts are enriched near telomeres (<100 Kb), over 80% of all interactions occurred >5 Mb away from chromosome ends. This isn't just about the subtelomere.

Our initial goal was to map telomeric regulation of key genes, like TERT. The reality? Gene-level interactions were massively outnumbered by contacts with repetitive elements! 🤯

To map the telomeric interactome, we developed Telomere-C (Telomere Conformation Capture). This novel, ligation-free method uses a biotinylated PNA probe to capture telomeric DNA, allowing us to measure nuclear contacts efficiently using short-read sequencing.

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Fully on board with this as you know and happy to contribute my 2 cents. Coordinates/position, be gone!

P.s. this is a repost of a deleted post from earlier today, where the link to the pre-print was not working. An update to BlueSky allowing post editing would be welcomed :)

This was pre-print number two of 2025 from our lab. Several more scheduled for public release later this year... Stay tuned!!

We're actively developing a novel high-sensitive method for #cfDNA analysis and anticipate a preprint on that approach very soon. Exciting times ahead for #LiquidBiopsy in #GBM research! Kudo's to @tgenresearch.bsky.social for supporting this work and #PrecisionMedicine in general!

This preprint marks a significant milestone for our lab and especially for Sharvari. Her dedication and scientific rigor shine through in this comprehensive study. Incredibly proud of her accomplishments and grateful for the opportunity to mentor her! 👩‍🔬

What's next? @sharvarimankame.bsky.social is currently evaluating cfDNA in plasma and CSF samples from #Glioblastoma patients, translating these exciting in vitro findings to the clinic.