Floris Barthel
@florisbarthel.bsky.social
Assistant professor @TGen | Postdoc @jacksonlab @MDAndersonNews | Genomics | Cancer | Gliomas | Telomeres
Yi-An Chen presenting his work on the telomeric interactome #ASHG2025, now also on biorxiv: www.biorxiv.org/content/10.1...
October 17, 2025 at 7:51 PM
Yi-An Chen presenting his work on the telomeric interactome #ASHG2025, now also on biorxiv: www.biorxiv.org/content/10.1...
Ogechukwu (Mimi) Mbegbu, super star RA and resident FISH-ing expert showcasing her cytogenetic analysis of astrocytes undergoing telomere dysfunction at #ASHG2025. Lots of chromosomal chaos observed and a 3-fold bias towards acrocentric abnormalities?! Stay tuned for the mechanism
October 16, 2025 at 9:44 PM
Ogechukwu (Mimi) Mbegbu, super star RA and resident FISH-ing expert showcasing her cytogenetic analysis of astrocytes undergoing telomere dysfunction at #ASHG2025. Lots of chromosomal chaos observed and a 3-fold bias towards acrocentric abnormalities?! Stay tuned for the mechanism
We validated these contacts with classic 3C/PCR and confirmed the 3D proximity using Southern Blotting and stunning 3D FISH data, proving these are real, higher-order chromatin structures.
October 13, 2025 at 11:21 PM
We validated these contacts with classic 3C/PCR and confirmed the 3D proximity using Southern Blotting and stunning 3D FISH data, proving these are real, higher-order chromatin structures.
Specifically, we found strong enrichment at three classes of repetitive elements: Interstitial Telomeric Sequences (ITS), Telomere-Associated Repeat 1 (TAR1), and D20S16 elements.
October 13, 2025 at 11:21 PM
Specifically, we found strong enrichment at three classes of repetitive elements: Interstitial Telomeric Sequences (ITS), Telomere-Associated Repeat 1 (TAR1), and D20S16 elements.
The telomeric interactome spans the entire genome! While contacts are enriched near telomeres (<100 Kb), over 80% of all interactions occurred >5 Mb away from chromosome ends. This isn't just about the subtelomere.
October 13, 2025 at 11:21 PM
The telomeric interactome spans the entire genome! While contacts are enriched near telomeres (<100 Kb), over 80% of all interactions occurred >5 Mb away from chromosome ends. This isn't just about the subtelomere.
Our initial goal was to map telomeric regulation of key genes, like TERT. The reality? Gene-level interactions were massively outnumbered by contacts with repetitive elements! 🤯
October 13, 2025 at 11:21 PM
Our initial goal was to map telomeric regulation of key genes, like TERT. The reality? Gene-level interactions were massively outnumbered by contacts with repetitive elements! 🤯
To map the telomeric interactome, we developed Telomere-C (Telomere Conformation Capture). This novel, ligation-free method uses a biotinylated PNA probe to capture telomeric DNA, allowing us to measure nuclear contacts efficiently using short-read sequencing.
October 13, 2025 at 11:21 PM
To map the telomeric interactome, we developed Telomere-C (Telomere Conformation Capture). This novel, ligation-free method uses a biotinylated PNA probe to capture telomeric DNA, allowing us to measure nuclear contacts efficiently using short-read sequencing.