All data are openly available:
– Explore at syndive.org

– RNA-seq at NCBI SRA PRJNA1142450 + PRJNA1142761

– Proteomics at PRIDE PXD054879

We hope this atlas will be a lasting community resource for studies of hippocampal biology, plasticity, and disease.

We combined RNAseq & proteomics with synapse-level resolution to map the hippocampus. We find thousands of molecules with spatial enrichment, reveal how protein half-life and local translation shape compartment identity and show that distal dendrites rely more strongly on local protein synthesis.

We hope this multi-omics atlas serves as a valuable resource for the neuroscience and multiomics community. Explore the data here: RNA-seq: PRJNA1142450 & PRJNA1142761 (NCBI SRA) and Proteomics: PRIDE PXD054879 @pride-ebi.bsky.social

Overall, this multi-omic study provides a comprehensive molecular map of hippocampus, revealing spatial gradients of transcripts and proteins, insights into mechanisms underlying synaptic diversity. The dataset is available for interactive exploration at syndive.org

5/Our FASS-based synaptic profiling revealed distinct transcriptomic and proteomic signatures across hippocampal subregions and strata. Synapse diversity is shaped by both input origin and local molecular composition, defining layer-specific plasticity and organization.

4/ Local translation versus trafficking emerged as a key mechanism shaping protein distribution, particularly in distal dendrites within the SLM, which might indicate a hotspot for locally synthesized proteins.

3/By integrating mRNA and protein data, we found that some proteins are tightly linked to local mRNA availability, while others are decoupled due to differences in protein half-lives.

2/ We dissected 3 subregions (CA1, CA3, DG) and 4 CA1 strata (SO, SP, SR, SLM) to profile >17,000 transcripts and ~10,000 proteins, capturing thousands of locally enriched molecules across receptors, channels, metabolism & adhesion families.

1/ Understanding brain molecular diversity requires mapping both mRNAs & proteins across regions & compartments. We combined microdissection, FASS-based synaptosome sorting, RNA-seq, and LC-MS/MS to do just that.

Absolutely, the omics world really is an exciting space to be in!

Thank you so much! Excited for all of us to keep exploring multiomics research🧠