5/Our FASS-based synaptic profiling revealed distinct transcriptomic and proteomic signatures across hippocampal subregions and strata. Synapse diversity is shaped by both input origin and local molecular composition, defining layer-specific plasticity and organization.

4/ Local translation versus trafficking emerged as a key mechanism shaping protein distribution, particularly in distal dendrites within the SLM, which might indicate a hotspot for locally synthesized proteins.

1/ Understanding brain molecular diversity requires mapping both mRNAs & proteins across regions & compartments. We combined microdissection, FASS-based synaptosome sorting, RNA-seq, and LC-MS/MS to do just that.

Excited to share our latest collaborative work from Erin Schuman's lab @mpibrain.bsky.social : An integrated transcriptomic and proteomic map of the mouse hippocampus at synaptic resolution, now published in @natcomms.nature.com. Here’s a thread summary 🧵
doi.org/10.1038/s414...