Dr. Märt-Erik Mäeots
@erikmaeots.bsky.social
Post doc developing time-resolved cryo-EM methods and AI models for cryo-EM optimization.
We combine all our tech into a data-driven workflow to optimize bespoke sample prep conditions per sample, at minimal additional effort for the user. Our next goal will be to make our system more widely available to everyone. #cryoEM
April 23, 2025 at 2:27 PM
We combine all our tech into a data-driven workflow to optimize bespoke sample prep conditions per sample, at minimal additional effort for the user. Our next goal will be to make our system more widely available to everyone. #cryoEM
We show a quantitative approach to cryo-EM sample preparation using high-speed cameras. We quantify ice thickness on *full* atlases in the TEM. We then use ‘manual gradient descent’ to improve our sample preparation method and try all sorts of things. In total, we made 2000 grids for this project!
April 23, 2025 at 2:25 PM
We show a quantitative approach to cryo-EM sample preparation using high-speed cameras. We quantify ice thickness on *full* atlases in the TEM. We then use ‘manual gradient descent’ to improve our sample preparation method and try all sorts of things. In total, we made 2000 grids for this project!
Time-resolved cryo-EM! We captured the earliest intermediates of homologous recombination in RecA with known lifetimes in milliseconds. We show that trEM can capture an incredible number of transient states, far beyond what we had hoped starting this project. www.biorxiv.org/content/10.1...
April 23, 2025 at 2:21 PM
Time-resolved cryo-EM! We captured the earliest intermediates of homologous recombination in RecA with known lifetimes in milliseconds. We show that trEM can capture an incredible number of transient states, far beyond what we had hoped starting this project. www.biorxiv.org/content/10.1...