Thank you!!

Thanks to the reviewers for their thoughtful feedback, @cp-cellreports.bsky.social for a great publishing experience, and @toettch.bsky.social and all our coauthors for making this such a fun project.

The power of pYtags is that they can readily be applied to any RTK of interest (and not just in Drosophila). I’d love to connect if you are interested in making a pYtag for your favorite RTK!

We built pYtags to detect RTK activity in Drosophila embryos and used them to learn about how developmental patterns form bsky.app/profile/emko...

So cool! Congrats Susanna!!

Thanks Ellen!

Thanks Tim!!

Possibly! If the phosphorylated substrate was membrane localized and could be tagged with the ITAM. Jak/Stat receptors would be a good example

I’ll be starting my undergrad-powered lab this summer at Claremont McKenna College and I’m super excited to continuing exploring new signaling biology with pYtags. If you are interested in tagging your favorite RTK (in flies or in other model systems), please reach out!

Huge thanks to: @toettch.bsky.social for being an awesome advisor. Payam for developing pYtags and starting this project with me. @rpkimyip.bsky.social and @eposfai.bsky.social for amazing egg chamber imaging. Alison and Stas for gorgeous trachea imaging. And Harrison who can segment anything!

Our data is consistent with a new conceptual model for terminal patterning where a local domain of Torso activity, tuned by negative feedback, produces a long-range gradient of ERK. Check out the paper to learn more!

Second, Torso activity was much more restricted to the poles than the broad gradient of ERK activity, consistent with a model where the ERK gradient forms via diffusion. We previously showed the same thing with an optogenetic stimulus.

journals.biologists.com/dev/article/...