We have had multiple discussions about why people have not previously observed the very dramatic reduction in transcription upon PNUTS depletion. So I looked at our own TT-seq data and analysed it without including the spike-in. The numbers speak for themselves! Spike-in calibration is important!

Go team PNUTS! @jesskelley.bsky.social @aleksszczurek.bsky.social @neilblackledge.bsky.social @robklose.bsky.social and Maciek!