Robert Hardt
@drrohardt.bsky.social
Senior mass spectrometry proteomics guy from Bonn, Germany. Works at Seer STAC Europe lab. Besides doing science loves rowing, running movies, comics, nerd stuff and spending quality time with his wife. Opinions here are independent of my employer.
My guess is that they used the tool from the Mann’s lab to optimize window placement to get the py-diaAiD PASEF method. Bit sketchy though.
July 24, 2025 at 6:01 PM
My guess is that they used the tool from the Mann’s lab to optimize window placement to get the py-diaAiD PASEF method. Bit sketchy though.
So they made it public. Nice
July 23, 2025 at 6:52 AM
So they made it public. Nice
Thanks for the prompt reply.
July 22, 2025 at 6:55 PM
Thanks for the prompt reply.
Was this done via unbiased shotgun LC-MS proteomics or target MS?
July 22, 2025 at 6:46 PM
Was this done via unbiased shotgun LC-MS proteomics or target MS?
Yes. At least whenever I did a submission in PRIDE the data is initially always private. The author has to actively click a publish button and then is also asked to provide reference to the publication the data is linked to. Usually DOI.
July 22, 2025 at 6:38 PM
Yes. At least whenever I did a submission in PRIDE the data is initially always private. The author has to actively click a publish button and then is also asked to provide reference to the publication the data is linked to. Usually DOI.
The paper mentions that the raw data will be deposited under: JPST003404/PXD056519. Probably since it’s freshly accepted the authors just have not made it public yet.
July 22, 2025 at 1:48 PM
The paper mentions that the raw data will be deposited under: JPST003404/PXD056519. Probably since it’s freshly accepted the authors just have not made it public yet.
Same here. I used the unadjusted p-value for y-axis plotting in the Volcano but drew the cutoff line based on p adjust.
February 5, 2025 at 6:30 PM
Same here. I used the unadjusted p-value for y-axis plotting in the Volcano but drew the cutoff line based on p adjust.
December 4, 2024 at 1:36 PM
resulting in an approach with both whole proteome coverage and high sensitivity for low abundant lysosomal hydrolases. Application for the analysis of a mouse model of metachromatic leukodystrohy resulted in exciting new insights in disease pathology. Check it out!
December 4, 2024 at 1:32 PM
resulting in an approach with both whole proteome coverage and high sensitivity for low abundant lysosomal hydrolases. Application for the analysis of a mouse model of metachromatic leukodystrohy resulted in exciting new insights in disease pathology. Check it out!
You will need to ask for a free viewer only license which has to be renewed every year unfortunately.
December 2, 2024 at 10:54 PM
You will need to ask for a free viewer only license which has to be renewed every year unfortunately.
I envy you. Also want one. 😜Would probably look also great as an ornament on the Christmas tree.
November 28, 2024 at 8:45 AM
I envy you. Also want one. 😜Would probably look also great as an ornament on the Christmas tree.
Congrats and all the best.
November 28, 2024 at 8:39 AM
Congrats and all the best.
But even longer times it is quite stable. We are using IC calibration at the start of each run as well.
November 26, 2024 at 11:15 PM
But even longer times it is quite stable. We are using IC calibration at the start of each run as well.
Roughly once a week.
November 26, 2024 at 11:13 PM
Roughly once a week.
Hi Lee. I could ask a friend of mine from the Netherlands who is a assistant prof in glycoproteomics. She is not on Bluesky yet though.
November 20, 2024 at 2:27 PM
Hi Lee. I could ask a friend of mine from the Netherlands who is a assistant prof in glycoproteomics. She is not on Bluesky yet though.