Katie Downes
@dr-downes.bsky.social
Post Doc @ Francis Crick Institute and UCL - COPII, CryoCLEM, cats, mammalian secretory pathway, image analysis
This revolutionised our segmentation of coated membranes.
Membrain V1 didn't recognise coated membranes - lots of manual editing was needed. Membrain V2 - beautiful segmentation, chefs kiss!
Membrain V1 didn't recognise coated membranes - lots of manual editing was needed. Membrain V2 - beautiful segmentation, chefs kiss!
June 16, 2025 at 10:55 AM
This revolutionised our segmentation of coated membranes.
Membrain V1 didn't recognise coated membranes - lots of manual editing was needed. Membrain V2 - beautiful segmentation, chefs kiss!
Membrain V1 didn't recognise coated membranes - lots of manual editing was needed. Membrain V2 - beautiful segmentation, chefs kiss!
Yes! we reproducibly get ~150nm thin lamella with very few failures and regularly run sessions for ~48hrs.
April 28, 2025 at 3:26 PM
Yes! we reproducibly get ~150nm thin lamella with very few failures and regularly run sessions for ~48hrs.
We have the Aquilos2 which is fab!
Our only complaint is the iFLM filter cubes are optimised for room temperature but the spectra of a number of fluorophores changes at cryo and so their peak excitation and emission wavelengths are filtered out leading to poor signal at cryo vs RT
Our only complaint is the iFLM filter cubes are optimised for room temperature but the spectra of a number of fluorophores changes at cryo and so their peak excitation and emission wavelengths are filtered out leading to poor signal at cryo vs RT
April 28, 2025 at 8:10 AM
We have the Aquilos2 which is fab!
Our only complaint is the iFLM filter cubes are optimised for room temperature but the spectra of a number of fluorophores changes at cryo and so their peak excitation and emission wavelengths are filtered out leading to poor signal at cryo vs RT
Our only complaint is the iFLM filter cubes are optimised for room temperature but the spectra of a number of fluorophores changes at cryo and so their peak excitation and emission wavelengths are filtered out leading to poor signal at cryo vs RT
@giuliazanetti.bsky.social something worth trying with our data maybe?
March 5, 2025 at 11:20 AM
@giuliazanetti.bsky.social something worth trying with our data maybe?
Both very well deserved!
December 19, 2024 at 3:08 PM
Both very well deserved!
We won’t be there I’m afraid, but we are always happy to chat!
November 27, 2024 at 4:51 PM
We won’t be there I’m afraid, but we are always happy to chat!
Could I please be added? 😇
November 27, 2024 at 3:32 PM
Could I please be added? 😇
👌 that cell placement 😍
November 26, 2024 at 11:58 AM
👌 that cell placement 😍
Honestly no idea how this happened. The training data looked fine! 🤯
November 25, 2024 at 12:40 PM
Honestly no idea how this happened. The training data looked fine! 🤯