Sebastian Bruchmann
@doktorbasti.bsky.social
Assistant Research Professor @ University of Cambridge / HLRI
Functional Genomics, AMR and Host Pathogen Interactions of Klebsiella pneumoniae and Pseudomonas aeruginosa
https://sebbruchmann.github.io/
Functional Genomics, AMR and Host Pathogen Interactions of Klebsiella pneumoniae and Pseudomonas aeruginosa
https://sebbruchmann.github.io/
I know of an untouched copy of the library here in Cambridge. It should be possible to access it but can't promise anything, unfortunately. Please drop me an email if you're interested in it - shgb2 at cam.ac.uk
University of Cambridge
The University of Cambridge is one of the world’s leading universities, with a rich history of radical thinking dating back to 1209.
cam.ac.uk
November 3, 2025 at 4:13 PM
I know of an untouched copy of the library here in Cambridge. It should be possible to access it but can't promise anything, unfortunately. Please drop me an email if you're interested in it - shgb2 at cam.ac.uk
The authors here present Dual Tn-seq, a method combining randomly barcoded transposon insertion sequencing (RB Tn-seq) with Cre-lox recombination, to generate and assay double mutants en masse.
The method looks great - congratulations to all involved!
The method looks great - congratulations to all involved!
September 26, 2025 at 11:42 AM
The authors here present Dual Tn-seq, a method combining randomly barcoded transposon insertion sequencing (RB Tn-seq) with Cre-lox recombination, to generate and assay double mutants en masse.
The method looks great - congratulations to all involved!
The method looks great - congratulations to all involved!
Transposon insertion sequencing (Tn-seq / TraDIS) lets us identify essential genes and, in principle, study genetic interactions (epistasis). But in bacteria, doing this at scale is hard.
September 26, 2025 at 11:42 AM
Transposon insertion sequencing (Tn-seq / TraDIS) lets us identify essential genes and, in principle, study genetic interactions (epistasis). But in bacteria, doing this at scale is hard.
I haven't done this in E. coli, but in Pseudomonas the attTn7 site via mini-Tn7 transposon integration worked well.
September 18, 2025 at 4:10 PM
I haven't done this in E. coli, but in Pseudomonas the attTn7 site via mini-Tn7 transposon integration worked well.
Wow - that is amazing! Many, many congratulations 👏🥳
June 18, 2025 at 10:12 AM
Wow - that is amazing! Many, many congratulations 👏🥳
Many, many congratulations Caity - so excited for you 🎉🥳
May 27, 2025 at 3:11 PM
Many, many congratulations Caity - so excited for you 🎉🥳
Many congratulations! 🎉
I've been using them for a while now and still can't believe how bright they are. Fantastic work! 👏
I've been using them for a while now and still can't believe how bright they are. Fantastic work! 👏
April 24, 2025 at 12:07 PM
Many congratulations! 🎉
I've been using them for a while now and still can't believe how bright they are. Fantastic work! 👏
I've been using them for a while now and still can't believe how bright they are. Fantastic work! 👏