Thanks for invitation again.

Thanks, and see you soon.

This method not only saves time, but is also economical, more efficient and mild in isolation conditions, preserving translation complexes, ribosome associated proteins and enables isolation of ribosomes for structural (cryo-EM) and functional studies. More on RAPPL uses still to come...

Nice work

My suggestion to everyone is to keep some of your most valuable cell lines and back-ups in your collaborators freezers or at least in different buildings or towns.

I should point out that this was a valuable collection of mutant cell lines as well as CRISPR/Cas9 engineered lines with endogenously tagged-RBPs, translation factors, ribosomal proteins and other proteins.