Douglas W. Houston
@dhouston.bsky.social
Developmental Biologist. Professor, #UIowa
Director, Developmental Studies Hybridoma Bank,
@dshb-antibodies.bsky.social
Views my own
Director, Developmental Studies Hybridoma Bank,
@dshb-antibodies.bsky.social
Views my own
Thanks to the folks at @HCRimaging for their nifty HCR HiFi encoders: "quantitative signal amplification of
1˚ antibodies for same-isotype multiplexing"
🔑@DSHB_antibodies can be tagged as supernatant, allowing mulplx wo/purification --> here are 2 trusted IgG1s used together
1˚ antibodies for same-isotype multiplexing"
🔑@DSHB_antibodies can be tagged as supernatant, allowing mulplx wo/purification --> here are 2 trusted IgG1s used together
![low-mag (5x) lateral view of a Xenopus tadpole; magenta is anti-skeletal muscle marker [12/101] staining the somites; green is anti-keratan sulfate [MZ15] staining notochord, both mouse IgG1s labeled with 546 and 647 encoders, respectively. Semi-cleared in 80% TDE.](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:drik3i6j2tqjkkocsdcu2p4l/bafkreiaad7bvjbckxkzk6hdqldmca2jxc2lm4jha5ibc7pkf2ewzryeje4@jpeg)
November 7, 2025 at 12:29 AM
Thanks to the folks at @HCRimaging for their nifty HCR HiFi encoders: "quantitative signal amplification of
1˚ antibodies for same-isotype multiplexing"
🔑@DSHB_antibodies can be tagged as supernatant, allowing mulplx wo/purification --> here are 2 trusted IgG1s used together
1˚ antibodies for same-isotype multiplexing"
🔑@DSHB_antibodies can be tagged as supernatant, allowing mulplx wo/purification --> here are 2 trusted IgG1s used together
Move-in day at Wake Forest … our youngest is off to college!
August 20, 2025 at 8:18 PM
Move-in day at Wake Forest … our youngest is off to college!
50 years later, hybridomas and monoclonal antibodies are still of "general interest" @dshb-antibodies.bsky.social
August 7, 2025 at 5:55 PM
50 years later, hybridomas and monoclonal antibodies are still of "general interest" @dshb-antibodies.bsky.social
...here's a nice example against fgf8!
June 18, 2025 at 3:36 PM
...here's a nice example against fgf8!
... plus see some nice work with @HCRimaging
on whole-mount chromogenic HCR in Xenopus on Sat.
on whole-mount chromogenic HCR in Xenopus on Sat.
June 18, 2025 at 3:36 PM
... plus see some nice work with @HCRimaging
on whole-mount chromogenic HCR in Xenopus on Sat.
on whole-mount chromogenic HCR in Xenopus on Sat.
June 18, 2025 at 3:36 PM
25 years since officially receiving my PhD (University of Miami*)-- the ~ 5 years between then and starting my first (only) faculty position feels very brief in retrospect.
* I still have the little laminated diploma they give out somewhere
* I still have the little laminated diploma they give out somewhere
January 31, 2025 at 10:11 PM
25 years since officially receiving my PhD (University of Miami*)-- the ~ 5 years between then and starting my first (only) faculty position feels very brief in retrospect.
* I still have the little laminated diploma they give out somewhere
* I still have the little laminated diploma they give out somewhere
It says ‘I voted’ — confident decency and sanity will prevail @KamalaHarris
January 31, 2025 at 10:11 PM
It says ‘I voted’ — confident decency and sanity will prevail @KamalaHarris
Xenopus making an appearance in our Neuroscience Highlights brochure! E7 is a great mAb against beta-tubulin and can be used to label neurons. #SfN24 @DSHB_antibodies
January 31, 2025 at 10:11 PM
Xenopus making an appearance in our Neuroscience Highlights brochure! E7 is a great mAb against beta-tubulin and can be used to label neurons. #SfN24 @DSHB_antibodies
Good luck to everyone setting up labs this fall.
Here's mine 20 years ago when I started @uiowabiology
(* lab web site still under construction)
Here's mine 20 years ago when I started @uiowabiology
(* lab web site still under construction)
January 31, 2025 at 10:11 PM
Good luck to everyone setting up labs this fall.
Here's mine 20 years ago when I started @uiowabiology
(* lab web site still under construction)
Here's mine 20 years ago when I started @uiowabiology
(* lab web site still under construction)
stocks replenished…
January 31, 2025 at 10:11 PM
stocks replenished…
College dropoff day for my eldest @GrinnellCollege … presided over by a giant squirrel.
Good luck #classof2028!
Good luck #classof2028!
January 31, 2025 at 10:11 PM
College dropoff day for my eldest @GrinnellCollege … presided over by a giant squirrel.
Good luck #classof2028!
Good luck #classof2028!
Find me at poster A1 and ask about new initiatives @DSHB_antibodies ! #2024SDB
January 31, 2025 at 10:12 PM
Find me at poster A1 and ask about new initiatives @DSHB_antibodies ! #2024SDB
Look who I ran into…
January 31, 2025 at 10:12 PM
Look who I ran into…
Scottish trains
January 31, 2025 at 10:12 PM
Scottish trains
‘Great chieftain o' the pudding-race!’
January 31, 2025 at 10:12 PM
‘Great chieftain o' the pudding-race!’
Different pub, slightly better result
January 31, 2025 at 10:12 PM
Different pub, slightly better result
A pleasant morning spent in the toponymic Village of Houston 🏴. Coffee at the old smithy IG:curlycoffeecompany and wandering through town. Houston House not public but caught a glimpse of its towers through the trees.
January 31, 2025 at 10:12 PM
A pleasant morning spent in the toponymic Village of Houston 🏴. Coffee at the old smithy IG:curlycoffeecompany and wandering through town. Houston House not public but caught a glimpse of its towers through the trees.
Cheers from a packed house in Glasgow for Scotland’s short-handed goal!
(* in a German bier hall but all tartan army fans)🏴
(* in a German bier hall but all tartan army fans)🏴
January 31, 2025 at 10:12 PM
Cheers from a packed house in Glasgow for Scotland’s short-handed goal!
(* in a German bier hall but all tartan army fans)🏴
(* in a German bier hall but all tartan army fans)🏴
Stebbins Cold Canyon trail, with Lake Berryessa (nor cal). Solvitur ambulando.
January 31, 2025 at 10:12 PM
Stebbins Cold Canyon trail, with Lake Berryessa (nor cal). Solvitur ambulando.
This was a very productive and enjoyable @YCharOS1 event last week in Montreal; antibody suppliers, researchers, funders, and data providers gathered to chart a path for more extensive #OpenScience antibody characterization.
January 31, 2025 at 10:12 PM
This was a very productive and enjoyable @YCharOS1 event last week in Montreal; antibody suppliers, researchers, funders, and data providers gathered to chart a path for more extensive #OpenScience antibody characterization.
Great name for a hotel bar. Not a random discovery this time …. it’s in my hotel!
January 31, 2025 at 10:12 PM
Great name for a hotel bar. Not a random discovery this time …. it’s in my hotel!
Back in my own course, a slight change of gear
January 31, 2025 at 10:12 PM
Back in my own course, a slight change of gear
also fun playing with transgenics from @XenopusNXR,
Nieuwkoop conjugates with muscle actin:GFP in the cap (only one of four had transgene though):
Nieuwkoop conjugates with muscle actin:GFP in the cap (only one of four had transgene though):
January 31, 2025 at 10:12 PM
also fun playing with transgenics from @XenopusNXR,
Nieuwkoop conjugates with muscle actin:GFP in the cap (only one of four had transgene though):
Nieuwkoop conjugates with muscle actin:GFP in the cap (only one of four had transgene though):