Dave Truong
@davidmtruong.bsky.social
Assistant Professor of Biomedical Engineering @NYU Tandon School of Engineering | Synthetic Biology lab | Genome re-writing in human iPSCs | Programmable off-the-shelf myeloid cell therapies | Regenerative tissue engineering.
www.truonglab.com
www.truonglab.com
The era of genome writing in human stem cells is here!
bsky.app/profile/davi...
bsky.app/profile/davi...
🚨 Preprint Alert!
The era of human genome writing begins…
We developed REWRITE for human iPSCs, enabling 100–209 kb rewriting of all 3 HLA genes - or anywhere.
➡️ On-demand HLA hiPSCs
➡️ Off-the-shelf cells
➡️ Extensible cell engineering
🧵⬇️
www.biorxiv.org/content/10.1...
The era of human genome writing begins…
We developed REWRITE for human iPSCs, enabling 100–209 kb rewriting of all 3 HLA genes - or anywhere.
➡️ On-demand HLA hiPSCs
➡️ Off-the-shelf cells
➡️ Extensible cell engineering
🧵⬇️
www.biorxiv.org/content/10.1...
Genomic REWRITE of Immune Interfaces Reveals Heritable Epigenetic Memory in Human Stem Cells
Human leukocyte antigen (HLA) polymorphism hinders allogeneic therapies. We developed REWRITE, a modular platform for iterative, scar-minimized genome writing of synthetic constructs >100 kb in human ...
www.biorxiv.org
September 17, 2025 at 2:08 PM
The era of genome writing in human stem cells is here!
bsky.app/profile/davi...
bsky.app/profile/davi...
This work was supported by
@niaidnews.bsky.social
@nyutandon.bsky.social
@newyorkuniversity.bsky.social
@nyulangone.bsky.social
@niaidnews.bsky.social
@nyutandon.bsky.social
@newyorkuniversity.bsky.social
@nyulangone.bsky.social
September 17, 2025 at 2:07 PM
This work was supported by
@niaidnews.bsky.social
@nyutandon.bsky.social
@newyorkuniversity.bsky.social
@nyulangone.bsky.social
@niaidnews.bsky.social
@nyutandon.bsky.social
@newyorkuniversity.bsky.social
@nyulangone.bsky.social
Critically, the final engineered cells are fully functional: they evade NK killing & establish T-cell tolerance.
This work provides a platform for programming complex human cells, from immune design to epigenetic rules.
This team effort was led by Serena Generoso &
Sarah Levovitz and many others!
This work provides a platform for programming complex human cells, from immune design to epigenetic rules.
This team effort was led by Serena Generoso &
Sarah Levovitz and many others!
September 17, 2025 at 2:06 PM
Critically, the final engineered cells are fully functional: they evade NK killing & establish T-cell tolerance.
This work provides a platform for programming complex human cells, from immune design to epigenetic rules.
This team effort was led by Serena Generoso &
Sarah Levovitz and many others!
This work provides a platform for programming complex human cells, from immune design to epigenetic rules.
This team effort was led by Serena Generoso &
Sarah Levovitz and many others!
This allowed us to ask: what controls this epigenetic ghost? By comparing a "genes-only" locus to one with native intergenic DNA, we found the answer.
This non-coding DNA contains the "software" that actively promotes silencing and resolves the 👻. 🧵
This non-coding DNA contains the "software" that actively promotes silencing and resolves the 👻. 🧵
September 17, 2025 at 2:05 PM
This allowed us to ask: what controls this epigenetic ghost? By comparing a "genes-only" locus to one with native intergenic DNA, we found the answer.
This non-coding DNA contains the "software" that actively promotes silencing and resolves the 👻. 🧵
This non-coding DNA contains the "software" that actively promotes silencing and resolves the 👻. 🧵
We built REWRITE to tackle a huge problem in cell therapy: HLA mismatch. We deleted massive regions and wrote in 100 kb synthetic haplotypes.
➡️iterative writing is possible
But this led to a surprise: the engineering left a heritable "epigenetic ghost" of active expression. 🧵
➡️iterative writing is possible
But this led to a surprise: the engineering left a heritable "epigenetic ghost" of active expression. 🧵
September 17, 2025 at 2:04 PM
We built REWRITE to tackle a huge problem in cell therapy: HLA mismatch. We deleted massive regions and wrote in 100 kb synthetic haplotypes.
➡️iterative writing is possible
But this led to a surprise: the engineering left a heritable "epigenetic ghost" of active expression. 🧵
➡️iterative writing is possible
But this led to a surprise: the engineering left a heritable "epigenetic ghost" of active expression. 🧵
Got any good ideas of what, other than lawsuits and protests? You're a leader in the community after all. I'm at a loss for what to do, especially from liberal NYC.
February 18, 2025 at 6:08 PM
Got any good ideas of what, other than lawsuits and protests? You're a leader in the community after all. I'm at a loss for what to do, especially from liberal NYC.