We then eliminated CT cells by using the NTR/MTZ #ablation system. After the specific elimination of CT cells, limb regeneration was delayed or truncated, underscoring the necessity of these cells for #regeneration 🤯

We defined the dynamic changes of cellular identities within the blastema over time by using 10X #Xenium spatial transcriptomics. Immune cells predominate in the first stages, whereas CT cells gradually increment and become the predominant cell type (~73%) at the late-bud blastema stage 🤔.

Pre-print alert:
This is our latest work on limb regeneration. We found that connective-tissue (CT) cells are necessary for this process by using a combinatorial approach of #spatial transcriptomics and an #ablation system for the first time in the #axolotl!
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www.biorxiv.org/content/10.1...