Bob Sclafani
@brewgenes.bsky.social
Professor Emeritus at the University of Colorado. Yeast Biologist. Home Brewer. Fermentation rules!
"You can observe a lot just by watching."-Yogi Berra
"You can observe a lot just by watching."-Yogi Berra
I have a few of those...LOL
November 26, 2025 at 5:58 PM
I have a few of those...LOL
PS Use the GST method if you don't have access to a BIOCORE machine
November 22, 2025 at 8:10 PM
PS Use the GST method if you don't have access to a BIOCORE machine
1. GST-puldown using recombinant proteins.
2. If you have purified proteins, use BIOCORE where you'll bet a kd.
2. If you have purified proteins, use BIOCORE where you'll bet a kd.
November 22, 2025 at 7:21 PM
1. GST-puldown using recombinant proteins.
2. If you have purified proteins, use BIOCORE where you'll bet a kd.
2. If you have purified proteins, use BIOCORE where you'll bet a kd.
I still have my copy
November 11, 2025 at 4:19 PM
I still have my copy
subway should make a commercial with this guy. How about, "I fought the law and my sandwich won"
November 7, 2025 at 1:32 AM
subway should make a commercial with this guy. How about, "I fought the law and my sandwich won"
He'll make it look like a cheap Bordello
October 31, 2025 at 10:29 PM
He'll make it look like a cheap Bordello
Quis custodiet ipsos custodes? (Who will watch the watchmen?) Roman poet Juvenal in 1-2nd century en.wikipedia.org/wiki/Quis_cu...
Quis custodiet ipsos custodes? - Wikipedia
en.wikipedia.org
October 27, 2025 at 8:25 PM
Quis custodiet ipsos custodes? (Who will watch the watchmen?) Roman poet Juvenal in 1-2nd century en.wikipedia.org/wiki/Quis_cu...
we had to read that paper for my first year prelim exam in grad school at Columbia.
October 5, 2025 at 9:54 PM
we had to read that paper for my first year prelim exam in grad school at Columbia.
Didn't Cairn's famous autoradiograph of the replicating E. coli chromosome take months of exposure?
September 12, 2025 at 9:52 PM
Didn't Cairn's famous autoradiograph of the replicating E. coli chromosome take months of exposure?
I find it interesting that these "Christians" always want to display the 10 commandments but not the Beatitudes (Matthew 5:1-12), in which Jesus blesses the poor, the meek, the hungry, the merciful, the peacemakers, etc. These are not blessed by these "Christians".
September 3, 2025 at 2:03 AM
I find it interesting that these "Christians" always want to display the 10 commandments but not the Beatitudes (Matthew 5:1-12), in which Jesus blesses the poor, the meek, the hungry, the merciful, the peacemakers, etc. These are not blessed by these "Christians".
I had the opposite but pleasing experience. I remembered experiments that they forgot but we're happy I did!
August 27, 2025 at 9:59 PM
I had the opposite but pleasing experience. I remembered experiments that they forgot but we're happy I did!
right. they all did good work
August 24, 2025 at 3:33 AM
right. they all did good work
and also by Ann Flower and Charles McHenry:Flower AM, McHenry CS. The gamma subunit of DNA polymerase III holoenzyme of Escherichia coli is produced by ribosomal frameshifting. Proc Natl Acad Sci U S A. 1990 May;87(10):3713-7. doi: 10.1073/pnas.87.10.3713. PMID: 2187190; PMCID: PMC53973.
August 23, 2025 at 8:54 PM
and also by Ann Flower and Charles McHenry:Flower AM, McHenry CS. The gamma subunit of DNA polymerase III holoenzyme of Escherichia coli is produced by ribosomal frameshifting. Proc Natl Acad Sci U S A. 1990 May;87(10):3713-7. doi: 10.1073/pnas.87.10.3713. PMID: 2187190; PMCID: PMC53973.
I think I went to this meeting in 1978, which had to be moved to UCLA at the last minute due to a snowstorm!
July 18, 2025 at 7:37 PM
I think I went to this meeting in 1978, which had to be moved to UCLA at the last minute due to a snowstorm!
I thought you would like this story as you're from Tennessee. I worked with a guy in the late 1960s who emigrated from Italy. He got drafted before that and was stationed in Tennessee. So he "learned" English there. Nobody could understand anything he said! LOL
July 5, 2025 at 4:35 PM
I thought you would like this story as you're from Tennessee. I worked with a guy in the late 1960s who emigrated from Italy. He got drafted before that and was stationed in Tennessee. So he "learned" English there. Nobody could understand anything he said! LOL
I did not know much about this until I saw the movie Mississippi Masala about an Indian family who was forced to leave Uganda by Idi Amin. I asked some of my Indian colleagues and got some amazing but sad stories. en.wikipedia.org/wiki/Mississ...
Mississippi Masala - Wikipedia
en.wikipedia.org
July 4, 2025 at 3:48 AM
I did not know much about this until I saw the movie Mississippi Masala about an Indian family who was forced to leave Uganda by Idi Amin. I asked some of my Indian colleagues and got some amazing but sad stories. en.wikipedia.org/wiki/Mississ...
I have had editors who did not "edit" and just keep giving you back what the reviewers said. When you are an editor, you need to reject bad reviews.
July 2, 2025 at 8:29 PM
I have had editors who did not "edit" and just keep giving you back what the reviewers said. When you are an editor, you need to reject bad reviews.
And there will be more sliminess. If Biden did this crap, they would impeach him.
May 27, 2025 at 10:08 PM
And there will be more sliminess. If Biden did this crap, they would impeach him.
Don't you think it's embarrassing when a Korean grad student has more guts than Columbia's trustees?
April 13, 2025 at 1:33 AM
Don't you think it's embarrassing when a Korean grad student has more guts than Columbia's trustees?
I have emailed the last 2 Columbia presidents and the Alumni assoc telling them to sue Trump. Why have they not? Is it because they're all corporate and not academic?
April 12, 2025 at 11:48 PM
I have emailed the last 2 Columbia presidents and the Alumni assoc telling them to sue Trump. Why have they not? Is it because they're all corporate and not academic?
Columbia needs to take legal action soon
March 19, 2025 at 3:24 AM
Columbia needs to take legal action soon