Boris Bogdanow
@bogdanow.bsky.social
Linking protein structure to function and mechanisms in virology. https://virologie-ccm.charite.de/forschung/ag_bogdanow
Curious about other viruses? Then also check out how SHVIP is applied to Influenza A virus infection of human cells and how it led to the identification of novel host factors. Pre-print with @jankosinski.bsky.social lab: doi.org/10.1101/2025...
Snapshot of in-cell protein contact sites reveals new host factors and hijacking of paraspeckles during influenza A virus infection
Influenza A virus (IAV) hijacks host cellular machinery, but many virus-IAV interactions and contacting protein sites remain uncharacterised, particularly those dependent on intact cellular architectu...
doi.org
July 22, 2025 at 8:15 AM
Curious about other viruses? Then also check out how SHVIP is applied to Influenza A virus infection of human cells and how it led to the identification of novel host factors. Pre-print with @jankosinski.bsky.social lab: doi.org/10.1101/2025...
A big integrative effort! XL-MS: Lars Mühlberg, @juliaruta.bsky.social and @fan-liu.bsky.social. Infectious work: Iris Gruska, Barbara Vetter and Lüder Wiebusch. Modeling: @bioinfo.se
July 22, 2025 at 8:15 AM
A big integrative effort! XL-MS: Lars Mühlberg, @juliaruta.bsky.social and @fan-liu.bsky.social. Infectious work: Iris Gruska, Barbara Vetter and Lüder Wiebusch. Modeling: @bioinfo.se
Why its relevant:
- It captures >700 PPIs and >6,000 cross-links
- It resolves compartment-specific interactions at the intact membrane system
- It integrates with AlphaFold to map domain-level PPIs, even in disordered regions
- It is validated by molecular virology + mutagenesis
- It captures >700 PPIs and >6,000 cross-links
- It resolves compartment-specific interactions at the intact membrane system
- It integrates with AlphaFold to map domain-level PPIs, even in disordered regions
- It is validated by molecular virology + mutagenesis
July 22, 2025 at 8:15 AM
Why its relevant:
- It captures >700 PPIs and >6,000 cross-links
- It resolves compartment-specific interactions at the intact membrane system
- It integrates with AlphaFold to map domain-level PPIs, even in disordered regions
- It is validated by molecular virology + mutagenesis
- It captures >700 PPIs and >6,000 cross-links
- It resolves compartment-specific interactions at the intact membrane system
- It integrates with AlphaFold to map domain-level PPIs, even in disordered regions
- It is validated by molecular virology + mutagenesis
SHVIP combines bio-orthogonal labeling during host shutoff and cross-linking mass-spec (XL-MS). XL-MS resolves proximity-based protein-protein interactions residue contact sites in situ and the bio-orthogonal labeling increases sensitivity of virus-specific interactome capture.
July 22, 2025 at 8:15 AM
SHVIP combines bio-orthogonal labeling during host shutoff and cross-linking mass-spec (XL-MS). XL-MS resolves proximity-based protein-protein interactions residue contact sites in situ and the bio-orthogonal labeling increases sensitivity of virus-specific interactome capture.