Birgitta Ryback
@birgitta-ryback.bsky.social
Small molecule LC/MS specialist. https://biryb.github.io/
I'm a creator and author at Bioanalytical Bees - tutorials and deep dives on all things LC/MS for the newbies and newbie-minded: https://bioanalytics.substack.com/
I'm a creator and author at Bioanalytical Bees - tutorials and deep dives on all things LC/MS for the newbies and newbie-minded: https://bioanalytics.substack.com/
This type of stuff always freaks me out! But it seems unlikely that the coumarin content would actually be on toxic levels
March 27, 2025 at 1:54 PM
This type of stuff always freaks me out! But it seems unlikely that the coumarin content would actually be on toxic levels
Thursday this week btw! Food>date with limited characters
March 25, 2025 at 8:33 PM
Thursday this week btw! Food>date with limited characters
I think they used both? Honestly I'm cool with MS1 ID (better than throwing out perfectly good features) especially if there's a validation experiment, but it should be with a reasonable tolerance and formula/rt matching. I tried to check the raw data but the DB didn't find the ID :/
March 24, 2025 at 2:35 PM
I think they used both? Honestly I'm cool with MS1 ID (better than throwing out perfectly good features) especially if there's a validation experiment, but it should be with a reasonable tolerance and formula/rt matching. I tried to check the raw data but the DB didn't find the ID :/
Top-4 DDA for a C18 separation leading to 631 differential metabolites? I do 2 complementary HILIC runs, top-15 DDA, and 600 _metabolites_ (not differential) is a 99th percentile result. I guess some of these annotations come from MS1 matching at that super generous 30 ppm allowable error?
March 21, 2025 at 9:25 PM
Top-4 DDA for a C18 separation leading to 631 differential metabolites? I do 2 complementary HILIC runs, top-15 DDA, and 600 _metabolites_ (not differential) is a 99th percentile result. I guess some of these annotations come from MS1 matching at that super generous 30 ppm allowable error?
I hope that's the case because alternatives are worse 😆 And agreed I expect more rigor from Cell Metabolism
March 14, 2025 at 3:09 PM
I hope that's the case because alternatives are worse 😆 And agreed I expect more rigor from Cell Metabolism
Do we still PubPeer? This is basically impossible
March 12, 2025 at 1:55 PM
Do we still PubPeer? This is basically impossible