Thanks Willow!

I don't really have one place you can see some stuff here:
github.com/mjrober101/3...
pubs.acs.org/doi/abs/10.1...

Have you tried using TPU for the membrane? That has been successful for me.

Case-by-case; but when I run 3DVA I pool the particles and do reconstructions. If particles around the two endpoints give nice maps of unique states but particles in the middle parts give degraded resolution map(s) that *look* like midpoint(s), I am wary of what the middle map(s) really are.

Discrete het. can be many types but for conformations some aspect of the transition is high energy with an insufficient population of particles to discern. Hot take: not always clear from things like 3DVA what's truly discrete vs truly continous vs. some kind of blend.

Continuous het. there is/are low energy barrier(s) between endpoints in a conformational transition & the data has robust populations of true particles in a full range of 'intermediates' along the path.

Could you add me please?

Could you please add me too?

Please add me as well!

My lab just started this year!

Really cool stuff! How are you liking the Bambu labs printer?