Why do we care? Because we have previously shown that UFC1 levels are negatively correlated with lean mass in various mouse strains, and knockdown of UFC1/UFMylation can increase muscle function. Maybe myosin UFMylation is a key link? elifesciences.org/articles/82951

We quantified UFMylation in the above human biopsies revealing several increased UFMylation sites including Myosins! Additional mapping of sites on Myosins reveal they are extensively modified, and in silico modelling suggest sites adjacent to ATP binding plays a functional role.

What did we find?... A whole bunch of cool substrates including UFMylation of Myosins which we validate following knockdown of UFC1 (the E2 ligase) in vivo combined with TMT-16plex labelling and LC-MS/MS…

In collaboration with CST, we developed antibodies that recognise "remnant ValGly" left on UFM1-conjugates following tryptic digestion. Similar to the remnant ubiquitin "diGly" approach published by www.cell.com/molecular-ce...

However, check out this anti-UFM1 blot from control muscle biopsies and people with ALS... A big band at 30kDa (probably RPL26) but clearly more to discover including big heavy bands >250kDa!

Hi #proteomics people, I'm hoping someone can help interpret these results. We have generated .fasta from RNAseq. We are testing the effects of different db size on min score cut-off @ 1% FDR. Hypothesis: as db increase, score increases. We don't see this with multiple algorithms and appears random.