Aydan Szeto
@aydanszeto.bsky.social
Immunologist @MRC-LMB | Views are my own | he/him 🏳️🌈 | I work towards manipulating the Yin and Yang of inflammation
#immunology #immunosky
#immunology #immunosky
Welcome to bluesky Sarah! Rod Rahimi assembled several immunology starter packs in case you’d like to connect with them :)
bsky.app/profile/rodr...
bsky.app/profile/rodr...
Friends, it's fun to see the #immunology community continue to grow! I created a 4th Starter Pack to help folks find each other. If you're an immunologist and you don't see yourself on the lists, let me know 🧪
go.bsky.app/PY5tCas
go.bsky.app/FmERUoD
go.bsky.app/6aT6qDS
go.bsky.app/QSEPDoj
go.bsky.app/PY5tCas
go.bsky.app/FmERUoD
go.bsky.app/6aT6qDS
go.bsky.app/QSEPDoj
Friends, the second #immunology starter pack filled in 3 days! I started a third to help folks new to BlueSky rebuild their network
Below are the links
If you're an immunologist and I have missed you so far, let me know 🧪
go.bsky.app/PY5tCas
go.bsky.app/FmERUoD
go.bsky.app/6aT6qDS
Below are the links
If you're an immunologist and I have missed you so far, let me know 🧪
go.bsky.app/PY5tCas
go.bsky.app/FmERUoD
go.bsky.app/6aT6qDS
December 2, 2024 at 9:24 PM
Welcome to bluesky Sarah! Rod Rahimi assembled several immunology starter packs in case you’d like to connect with them :)
bsky.app/profile/rodr...
bsky.app/profile/rodr...
Should we “normalise” the gut myco(& micro)biome across facilities to increase reproducibility? What is normal?
November 27, 2024 at 10:13 PM
Should we “normalise” the gut myco(& micro)biome across facilities to increase reproducibility? What is normal?
The authors further showed that mice from JAX lab (where many of us get lab mice from) lack this commensal. Differences in mycobiome can dramatically influence immunity.
November 27, 2024 at 10:13 PM
The authors further showed that mice from JAX lab (where many of us get lab mice from) lack this commensal. Differences in mycobiome can dramatically influence immunity.
In @nature, Liao et al showed this commensal promotes type-2 immunity in the gut via IL-33/ST2, influencing protective anti-helminth responses and food allergy models.
November 27, 2024 at 10:13 PM
In @nature, Liao et al showed this commensal promotes type-2 immunity in the gut via IL-33/ST2, influencing protective anti-helminth responses and food allergy models.
Happy to answer any specific questions you may have and/or share my experience either here or privately :)
November 24, 2024 at 4:39 PM
Happy to answer any specific questions you may have and/or share my experience either here or privately :)
We used BD Cytofix/perm, Foxp3 fix/perm or 2% PFA fixation and these were all compatible with 2W1S tetramer staining and downstream cytokine analyses by flow
November 24, 2024 at 3:06 PM
We used BD Cytofix/perm, Foxp3 fix/perm or 2% PFA fixation and these were all compatible with 2W1S tetramer staining and downstream cytokine analyses by flow
I can still see some after stimulation, so it’s not like the stimulation will completely negate tetramer staining (at least for 2W1S-MHCII tetramers). Worth a try!
November 24, 2024 at 2:46 PM
I can still see some after stimulation, so it’s not like the stimulation will completely negate tetramer staining (at least for 2W1S-MHCII tetramers). Worth a try!
Agree with the above and I will add that at least for class 2 tetramers, it will depend on the specific tetramers that you use. Unfortunately a lot of the class 2 tetramers are not great compared to class 1, and in my experience both the MFI and % positives may reduce after stimulation, but
November 24, 2024 at 2:46 PM
Agree with the above and I will add that at least for class 2 tetramers, it will depend on the specific tetramers that you use. Unfortunately a lot of the class 2 tetramers are not great compared to class 1, and in my experience both the MFI and % positives may reduce after stimulation, but
Can I be added please :)
November 18, 2024 at 9:17 PM
Can I be added please :)
Some of us already hallucinate more than others and we definitely don’t need more of that
November 18, 2024 at 5:15 PM
Some of us already hallucinate more than others and we definitely don’t need more of that