𝐀𝐯𝐞𝐫𝐲 𝐒𝐮𝐧
@averyruisun.bsky.social
Ph.D. candidate @jennyoung.bsky.social Lab @MBIsg.bsky.social @BME_NUS | 🔬🫀 Extracellular Matrix & Mechanobiology
Thank you Guillaume!!
June 30, 2025 at 5:31 AM
Thank you Guillaume!!
Thx for reading thus far, check out the full story now!
nature.com/articles/s4156…
Shout out to the Young Lab and our amazing collaborators Roger Foo, Yuchen Long, Jin Zhu, and Matt Ackers-Johnson. Teamwork makes the dream work 😻 @NUSMedicine @NUS_DBS
nature.com/articles/s4156…
Shout out to the Young Lab and our amazing collaborators Roger Foo, Yuchen Long, Jin Zhu, and Matt Ackers-Johnson. Teamwork makes the dream work 😻 @NUSMedicine @NUS_DBS
https://nature.com/articles/s4156…
June 13, 2025 at 2:17 PM
Thx for reading thus far, check out the full story now!
nature.com/articles/s4156…
Shout out to the Young Lab and our amazing collaborators Roger Foo, Yuchen Long, Jin Zhu, and Matt Ackers-Johnson. Teamwork makes the dream work 😻 @NUSMedicine @NUS_DBS
nature.com/articles/s4156…
Shout out to the Young Lab and our amazing collaborators Roger Foo, Yuchen Long, Jin Zhu, and Matt Ackers-Johnson. Teamwork makes the dream work 😻 @NUSMedicine @NUS_DBS
We found that ECM ligands and mechanics distinctly regulate fibroblast behavior. And ligands outweigh mechanics in dictating fibroblast activation and rejuvenation. We also predicted ECM- vs biophysical-therapy efficacy with DECIPHER. It’s applicable to other tissue/disease.
June 13, 2025 at 2:17 PM
We found that ECM ligands and mechanics distinctly regulate fibroblast behavior. And ligands outweigh mechanics in dictating fibroblast activation and rejuvenation. We also predicted ECM- vs biophysical-therapy efficacy with DECIPHER. It’s applicable to other tissue/disease.
DECIPHER: DECellularized In situ Polyacrylamide Hydrogel—Ecm hybRid, enabling independent stiffness tunability while maintaining native ECM composition, architecture, and tissue viscoelasticity, perfect for seeding cells afterwards.
June 13, 2025 at 2:17 PM
DECIPHER: DECellularized In situ Polyacrylamide Hydrogel—Ecm hybRid, enabling independent stiffness tunability while maintaining native ECM composition, architecture, and tissue viscoelasticity, perfect for seeding cells afterwards.
And thankfully, she was super supportive!!🥹 We met the next day and planned everything that needed optimization and everything that might go wrong along the way. A few months later, luckily, it worked very well, and we got many exciting findings from our new system: DECIPHER😆🈶
June 13, 2025 at 2:17 PM
And thankfully, she was super supportive!!🥹 We met the next day and planned everything that needed optimization and everything that might go wrong along the way. A few months later, luckily, it worked very well, and we got many exciting findings from our new system: DECIPHER😆🈶
I couldn’t sleep at all for the rest of the night—but for a different reason. I planned out in my head every experiment needed to test and optimize the idea to see if it works. And I prepared my pitch to Jennifer that I want to change my thesis almost entirely 🥺.
June 13, 2025 at 2:17 PM
I couldn’t sleep at all for the rest of the night—but for a different reason. I planned out in my head every experiment needed to test and optimize the idea to see if it works. And I prepared my pitch to Jennifer that I want to change my thesis almost entirely 🥺.
And our lab @jennyoung.bsky.social happens to know enough about polyacrylamide from Engler’s work that we might be able to decouple substrate stiffness in the meantime by controlling the hydrogel recipe ⁉️
June 13, 2025 at 2:17 PM
And our lab @jennyoung.bsky.social happens to know enough about polyacrylamide from Engler’s work that we might be able to decouple substrate stiffness in the meantime by controlling the hydrogel recipe ⁉️
This is a fundamentally different decellularization technique from how it’s been done for decades.
We used an interpenetrating polyacrylamide network to stabilize ECM before in situ decellularization. This keeps ECM in their native form (no pulverization or digestion).
We used an interpenetrating polyacrylamide network to stabilize ECM before in situ decellularization. This keeps ECM in their native form (no pulverization or digestion).
June 13, 2025 at 2:17 PM
This is a fundamentally different decellularization technique from how it’s been done for decades.
We used an interpenetrating polyacrylamide network to stabilize ECM before in situ decellularization. This keeps ECM in their native form (no pulverization or digestion).
We used an interpenetrating polyacrylamide network to stabilize ECM before in situ decellularization. This keeps ECM in their native form (no pulverization or digestion).
I learned it as a polyacrylamide-assisted tissue clearing platform, which removes lipid bilayers to enable whole-organ imaging 🔬. But as my mind ran wild in bed, I wondered—what if we took it a step further and removed cells entirely, instead of just the membranes? 🧐
June 13, 2025 at 2:17 PM
I learned it as a polyacrylamide-assisted tissue clearing platform, which removes lipid bilayers to enable whole-organ imaging 🔬. But as my mind ran wild in bed, I wondered—what if we took it a step further and removed cells entirely, instead of just the membranes? 🧐
One night I couldn’t sleep, pondering about my thesis and about this dilemma that me and this field is facing. And a paper that had nothing to do with this somehow came to my mind: CLARITY, developed by the Deisseroth lab at Stanford.
www.nature.com/articles/nat...
www.nature.com/articles/nat...
Structural and molecular interrogation of intact biological systems - Nature
High-resolution imaging has traditionally required thin sectioning, a process that disrupts long-range connectivity in the case of brains: here, intact mouse brains and human brain samples have been m...
www.nature.com
June 13, 2025 at 2:17 PM
One night I couldn’t sleep, pondering about my thesis and about this dilemma that me and this field is facing. And a paper that had nothing to do with this somehow came to my mind: CLARITY, developed by the Deisseroth lab at Stanford.
www.nature.com/articles/nat...
www.nature.com/articles/nat...
Interesting to think about! But we couldn’t find existing biomaterial systems that can answer such questions — classical reconstituted decellularized ECM loses mechanics, while hydrogels lacks mature ECM ligands…🥲
June 13, 2025 at 2:17 PM
Interesting to think about! But we couldn’t find existing biomaterial systems that can answer such questions — classical reconstituted decellularized ECM loses mechanics, while hydrogels lacks mature ECM ligands…🥲
We set out to answer a longstanding question regarding extracellular matrix: is the ECM influencing cells more via ligand biochemistry or mechanobiology?
🧪⚖️💪🏻
🧪⚖️💪🏻
June 13, 2025 at 2:17 PM
We set out to answer a longstanding question regarding extracellular matrix: is the ECM influencing cells more via ligand biochemistry or mechanobiology?
🧪⚖️💪🏻
🧪⚖️💪🏻
DECIPHER: DECellularized In situ Polyacrylamide Hydrogel—Ecm hybRid, enabling independent stiffness tunability while maintaining native ECM composition, architecture, and tissue viscoelasticity, perfect for seeding cells afterwards.
June 13, 2025 at 2:17 PM
DECIPHER: DECellularized In situ Polyacrylamide Hydrogel—Ecm hybRid, enabling independent stiffness tunability while maintaining native ECM composition, architecture, and tissue viscoelasticity, perfect for seeding cells afterwards.
And thankfully, she was super supportive!!🥹 We met the next day and planned everything that needed optimization and everything that might go wrong along the way. A few months later, luckily, it worked very well, and we got many exciting findings from our new system: DECIPHER😆🈶
June 13, 2025 at 2:17 PM
And thankfully, she was super supportive!!🥹 We met the next day and planned everything that needed optimization and everything that might go wrong along the way. A few months later, luckily, it worked very well, and we got many exciting findings from our new system: DECIPHER😆🈶
I couldn’t sleep at all for the rest of the night—but for a different reason. I planned out in my head every experiment needed to test and optimize the idea to see if it works. And I prepared my pitch to Jennifer that I want to change my thesis almost entirely 🥺.
June 13, 2025 at 2:17 PM
I couldn’t sleep at all for the rest of the night—but for a different reason. I planned out in my head every experiment needed to test and optimize the idea to see if it works. And I prepared my pitch to Jennifer that I want to change my thesis almost entirely 🥺.
And our lab @jennyoung.bsky.social happens to know enough about polyacrylamide from Engler’s work that we might be able to decouple substrate stiffness in the meantime by controlling the hydrogel recipe ⁉️
June 13, 2025 at 2:17 PM
And our lab @jennyoung.bsky.social happens to know enough about polyacrylamide from Engler’s work that we might be able to decouple substrate stiffness in the meantime by controlling the hydrogel recipe ⁉️
This is a fundamentally different decellularization technique from how it’s been done for decades.
We used an interpenetrating polyacrylamide network to stabilize ECM before in situ decellularization. This keeps ECM in their native form (no pulverization or digestion).
We used an interpenetrating polyacrylamide network to stabilize ECM before in situ decellularization. This keeps ECM in their native form (no pulverization or digestion).
June 13, 2025 at 2:17 PM
This is a fundamentally different decellularization technique from how it’s been done for decades.
We used an interpenetrating polyacrylamide network to stabilize ECM before in situ decellularization. This keeps ECM in their native form (no pulverization or digestion).
We used an interpenetrating polyacrylamide network to stabilize ECM before in situ decellularization. This keeps ECM in their native form (no pulverization or digestion).
I learned it as a polyacrylamide-assisted tissue clearing platform, which removes lipid bilayers to enable whole-organ imaging 🔬. But as my mind ran wild in bed, I wondered—what if we took it a step further and removed cells entirely, instead of just the membranes? 🧐
June 13, 2025 at 2:17 PM
I learned it as a polyacrylamide-assisted tissue clearing platform, which removes lipid bilayers to enable whole-organ imaging 🔬. But as my mind ran wild in bed, I wondered—what if we took it a step further and removed cells entirely, instead of just the membranes? 🧐
One night I couldn’t sleep, pondering about my thesis and about this dilemma that me and this field is facing. And a paper that had nothing to do with this somehow came to my mind: CLARITY, developed by the Deisseroth lab at Stanford.
www.nature.com/articles/nat...
www.nature.com/articles/nat...
Structural and molecular interrogation of intact biological systems - Nature
High-resolution imaging has traditionally required thin sectioning, a process that disrupts long-range connectivity in the case of brains: here, intact mouse brains and human brain samples have been m...
www.nature.com
June 13, 2025 at 2:17 PM
One night I couldn’t sleep, pondering about my thesis and about this dilemma that me and this field is facing. And a paper that had nothing to do with this somehow came to my mind: CLARITY, developed by the Deisseroth lab at Stanford.
www.nature.com/articles/nat...
www.nature.com/articles/nat...
Interesting to think about! But we couldn’t find existing biomaterial systems that can answer such questions — classical reconstituted decellularized ECM loses mechanics, while hydrogels lacks mature ECM ligands…🥲
June 13, 2025 at 2:17 PM
Interesting to think about! But we couldn’t find existing biomaterial systems that can answer such questions — classical reconstituted decellularized ECM loses mechanics, while hydrogels lacks mature ECM ligands…🥲
We set out to answer a longstanding question regarding extracellular matrix: is the ECM influencing cells more via ligand biochemistry or mechanobiology?
🧪⚖️💪🏻
🧪⚖️💪🏻
June 13, 2025 at 2:17 PM
We set out to answer a longstanding question regarding extracellular matrix: is the ECM influencing cells more via ligand biochemistry or mechanobiology?
🧪⚖️💪🏻
🧪⚖️💪🏻