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Lineage tracing of Eomes-positive mouse trophoblast cells confirms their capacity to contribute to both placental layers in vivo even after E7.5

-Avery McGinnis, Megan Cull, Nichole Peterson, Matthew Tang, Bryony Natale @bnatale.bsky.social, David Natale

#Extraembryonic
Immunofluorescence Staining of E17.5 Ai6 x Eomes-Cre Placentae for Mesenchymal Cells. EomesPOS cells and daughters (ZsGreen; green), all counterstained with NucBlue (DAPI; blue). (A) Staining of endothelial cells in the chorion and lower labyrinth layer with an anti-CD31 antibody (red; 400x). Ai6NEG (above) and Ai6POS (below). (B) Staining of placental pericytes in the labyrinth layer with an anti-αSMA antibody (red; 400x). Ai6NEG (above) and Ai6POS (below). White arrowheads show colocalization of ZsGreen with antibody staining.
October 6, 2025 at 11:43 AM