Astrocytes (🔵) and microglia (🟡) in the hippocampus of a mouse brain for #FluorescenceFriday

#GLIA2025 is right around the corner! Catch the poster presentations from Klaus-Armin Nave’s group and talk all things glia in health and in Alzheimer’s disease ✌🏼

@gliameeting.bsky.social

Throwback #FluorescenceFriday of large motor neurons filled with phospho-tau in the spinal cord of the PS19 mouse model.

🔵 for phospho-tau
🟡 for axons

Coronal view of a mouse brain lacking a major myelin protein (PLP 🟡) expression in the forebrain and some GFAP+ astrocytes (🔵).

Note the patchy leftover myelin in the recombined cortex and corpus callosum but not in the unrecombined striatum.

#FluorescenceFriday

Stoked to be part of this amazing selection of images 🤩

My entry is #3: “The glial response to amyloid skies” and I submitted this image because Aβ plaques and their local environment have always amazed me.

Vote here: focalplane.biologists.com/2025/03/11/v...

Some motor neurons (🔵) filled with phospho-tau (🟡) in the spinal cord of one of our PS19 crossbreeds. Taken together with @erinneuro.bsky.social.

#FluorescenceFriday

For #FluorescenceFriday, a side-by-side comparison of 5xFAD hemibrains at 6 months showing how Aβ plaques can be modulated not just by sex but also by the transgene parentage.

More details from our recent paper where we reported the transgene inheritance effect in 5xFAD mice 👇

Could 5xFAD mothers confer a protective immune priming effect to their offspring? Despite being carried by the same 5xFAD mothers, plaque load in the offspring of 5xFAD pairs still bifurcated into high and low levels. This rules out potential maternal immune priming effects. (8/11)

We then asked if the effect lasts for multiple generations by tracing the grandparental lineage. Mice inheriting the transgene from 2 consecutive maternal sources had the lowest plaque load, but this additive effect by the grandparental source is minimal compared to the parental effect. (6/11)

In fact, it does 🤯 Through LSM, we analyzed plaque burden in male 5xFAD mice with distinct parental sources for the transgene. Mice inheriting the transgene from the mother developed much lower plaque load (up to half in the cortex) than those with a paternal inheritance 🫢 (5/11)

We confirmed the previously reported sex dimorphism in this model, with female 5xFAD mice showing double the plaque burden of age-matched males. This is especially evident in the cortex, showcasing the lag in plaque deposition in the cortex compared to the hippocampus. (3/11)

First, we established that quantitative light-sheet microscopy (LSM) can reliably measure differences in plaque load. We demonstrated the age-related plaque increase in 5xFAD mice: plaque deposition occurs rapidly in the first 8 months, then numbers plateau over time. (2/11)

An array of microglia (🔵) and a neuron filled with phospho-tau (🟡) in the cortex of a PS19 mouse.

#FluorescenceFriday

A mouse hippocampus labeled for oligodendrocytes 🟡, myelin 🔵, and nuclei ⚪️. You can appreciate how the distribution of myelin and oligodendrocytes is not homogeneous in the overall hippocampal formation.

#FluorescenceFriday

A light-sheet image of a 6-month-old female 5xFAD mouse hemibrain labeled with Congo red 🔬 Take a look at the abundance of cortical and hippocampal Aβ plaques (white dots).

3D render on top and serial 2D sagittal sections below.

#FluorescenceFriday #AlzheimersDisease

Astrocytes (🟡) and myelin (🔵) in the corpus callosum of a mouse brain.

#FluorescenceFriday

Regarding these 3 studies showing that OLs contribute to Aβ plaque formation in AD mice, I still find it surreal how all 3 groups used almost exactly the same approach unbeknownst to each other (at first), performed similar pilot experiments, and came to complementing conclusions in the same year 😵‍💫

First #FluorescenceFriday here! A throwback image of a lone oligodendrocyte (🟡) contacting axons (🔵) with its many processes in a mouse hippocampus.

#neurosky #gliasky