Along with twitching, we also learned that combining flow and peroxide has a detrimental effect on cell physiology as they inhibit bacterial growth.

While looking at cells in flow, we serendipitously discovered that flow and peroxide synergise to block bacterial migration.

We discovered that natural levels of hydrogen peroxide induces a small, clearly defined transcriptional response. Comparative analysis revealed that studies using batch cultures overestimated the concentrations needed and genes induced by hydrogen peroxide.

Using microfluidics, we simultaneously provided host-relevant levels of flow and hydrogen peroxide to P. aeruginosa cells. The replenishing power of flow increases peroxide effectiveness 50-fold, allowing for natural levels of peroxide to block bacterial migration and growth.

Do you feel stressed?
Is it one thing or a combination?

Turns out bacteria can’t handle stress either!

Excited to share my first first author paper out now in @currentbiology.bsky.social!

Combining multiple stressors blocks bacterial migration and growth! www.cell.com/current-biol...