🧬 We demonstrate that long-read single-cell RNA-seq can enable direct immunoglobulin chain reconstruction and resolve isoform usage in immune cells, while also highlighting that limitations common across all platforms still constrain the complexity we can recover

To move forward, we need better benchmarking and pipelines that are optimised for the realities of single-cell data — sparse, noisy, and complex.

Otherwise, we risk missing the very biology we’re trying to uncover 🧬

#SingleCell #LongReads #IsoformResolution #Genomics #Bioinformatics

There’s a strong push in the field to maximise cell throughput — but meaningful isoform analysis RELIES on having enough resolution per cell 🔬

It’s a trade-off, and the risk is that depth gets overlooked - even though it’s exactly what’s needed to study splicing and capture transcript diversity.