Anita Donlic
@anitadonlic.bsky.social
Postdoc in Brangwynne lab at Princeton, alumna of Hargrove lab. Working at the intersection of RNA chemical biology and condensates. Originally from Bosnia & Herzegovina
Congratulations!! This is so exciting and well-deserved. 🎉
September 29, 2025 at 3:24 PM
Congratulations!! This is so exciting and well-deserved. 🎉
... Prof. Ai Ing Lim and @kristantunes.bsky.social
for a great help with the virus work, and Cliff @brangwynnelab.bsky.social for all the support and guidance!
for a great help with the virus work, and Cliff @brangwynnelab.bsky.social for all the support and guidance!
August 20, 2025 at 9:16 PM
... Prof. Ai Ing Lim and @kristantunes.bsky.social
for a great help with the virus work, and Cliff @brangwynnelab.bsky.social for all the support and guidance!
for a great help with the virus work, and Cliff @brangwynnelab.bsky.social for all the support and guidance!
To say that this took a village would be an understatement. I’m incredibly grateful to my collaborators in the @brangwynnelab.bsky.social : Troy Comi, @sofiquinodoz.bsky.social, @nima-jaberi.bsky.social, Lenny Weisner and Lifei Jiang, ...
August 20, 2025 at 9:16 PM
To say that this took a village would be an understatement. I’m incredibly grateful to my collaborators in the @brangwynnelab.bsky.social : Troy Comi, @sofiquinodoz.bsky.social, @nima-jaberi.bsky.social, Lenny Weisner and Lifei Jiang, ...
🌟 Big picture 🌟
✔️ Condensate morphology encodes function
✔️ Deep-Phase translates images into quantitative readouts of drug biochemical potency
✔️ Future applications: phenotypic screens, MoA studies, biomarkers, single-cell heterogeneity detection (10/10)
✔️ Condensate morphology encodes function
✔️ Deep-Phase translates images into quantitative readouts of drug biochemical potency
✔️ Future applications: phenotypic screens, MoA studies, biomarkers, single-cell heterogeneity detection (10/10)
August 20, 2025 at 9:16 PM
🌟 Big picture 🌟
✔️ Condensate morphology encodes function
✔️ Deep-Phase translates images into quantitative readouts of drug biochemical potency
✔️ Future applications: phenotypic screens, MoA studies, biomarkers, single-cell heterogeneity detection (10/10)
✔️ Condensate morphology encodes function
✔️ Deep-Phase translates images into quantitative readouts of drug biochemical potency
✔️ Future applications: phenotypic screens, MoA studies, biomarkers, single-cell heterogeneity detection (10/10)
Integrating our discoveries, we came up with a new model for nucleolar phase boundary maintenance, in which both the abundance and processing state of rRNA📊 dictate the multiphase arrangement within this fascinating condensate (9/10).
August 20, 2025 at 9:16 PM
Integrating our discoveries, we came up with a new model for nucleolar phase boundary maintenance, in which both the abundance and processing state of rRNA📊 dictate the multiphase arrangement within this fascinating condensate (9/10).
These observations led us to hypothesize that sequential cleavage inhibition💊 (invert & push apart DFCs from GC) followed by transcription inhibition💊 (restore DFC-GC interface) could phenocopy the flower 🌸 morphology. This is indeed what we (and Deep-Phase) saw! (8/10)
August 20, 2025 at 9:16 PM
These observations led us to hypothesize that sequential cleavage inhibition💊 (invert & push apart DFCs from GC) followed by transcription inhibition💊 (restore DFC-GC interface) could phenocopy the flower 🌸 morphology. This is indeed what we (and Deep-Phase) saw! (8/10)
We then conduct mechanistic studies 🔎 to find that beyond its recognized role in resolving supercoiling, TOP1 is also involved in rRNA processing! Its inhibition causes rRNA cleavage defects and a drop in transcription of large ribosomal subunit precursors ❌🏭. (7/10)
August 20, 2025 at 9:16 PM
We then conduct mechanistic studies 🔎 to find that beyond its recognized role in resolving supercoiling, TOP1 is also involved in rRNA processing! Its inhibition causes rRNA cleavage defects and a drop in transcription of large ribosomal subunit precursors ❌🏭. (7/10)
To test the utility of Deep-Phase, we conduct a small molecule screen and uncover a unique nucleolar morphology: the "flower" 🌸! This phenotype, where the DFC-GC interface is maintained but inverted, arises specifically upon DNA Topoisomerase I (TOP1) depletion. (6/10)
August 20, 2025 at 9:16 PM
To test the utility of Deep-Phase, we conduct a small molecule screen and uncover a unique nucleolar morphology: the "flower" 🌸! This phenotype, where the DFC-GC interface is maintained but inverted, arises specifically upon DNA Topoisomerase I (TOP1) depletion. (6/10)
Not just nucleoli! We extend Deep-Phase to nuclear splicing speckles✂️ as well as RSV viral cytoplasmic factories🦠, showing that condensate morphology alterations are a quantitative fingerprint of the degree of RNA biochemistry disruptions within them. (5/10)
August 20, 2025 at 9:16 PM
Not just nucleoli! We extend Deep-Phase to nuclear splicing speckles✂️ as well as RSV viral cytoplasmic factories🦠, showing that condensate morphology alterations are a quantitative fingerprint of the degree of RNA biochemistry disruptions within them. (5/10)
In doing so, we measure dose-response curves from images and demonstrate that they match IC50 values from biochemical experiments: nucleolar morphology becomes a quantitative readout of potencies of drugs that alter ribosome biogenesis! 📉➡️📈 (4/10)
August 20, 2025 at 9:16 PM
In doing so, we measure dose-response curves from images and demonstrate that they match IC50 values from biochemical experiments: nucleolar morphology becomes a quantitative readout of potencies of drugs that alter ribosome biogenesis! 📉➡️📈 (4/10)
We developed Deep-Phase, an automated imaging and deep neural network-based framework that accurately tracks these changes over treatment times⏲️and concentrations📶 directly from images (no hand-picked features) (3/10).
August 20, 2025 at 9:16 PM
We developed Deep-Phase, an automated imaging and deep neural network-based framework that accurately tracks these changes over treatment times⏲️and concentrations📶 directly from images (no hand-picked features) (3/10).
Cells organize key processes in biomolecular condensates, such as the multiphase nucleolus where ribosomes are made 🏭. Due to its dynamic and compartmentalized nature, small molecule perturbations of this process💊 lead to fast and distinct morphology rearrangements🌀. (2/10)
August 20, 2025 at 9:16 PM
Cells organize key processes in biomolecular condensates, such as the multiphase nucleolus where ribosomes are made 🏭. Due to its dynamic and compartmentalized nature, small molecule perturbations of this process💊 lead to fast and distinct morphology rearrangements🌀. (2/10)
Ai Ing Lim and @kristantunes.bsky.social for a great help with the virus work, and Cliff @brangwynnelab.bsky.social for all the support and guidance!
August 20, 2025 at 8:01 PM
Ai Ing Lim and @kristantunes.bsky.social for a great help with the virus work, and Cliff @brangwynnelab.bsky.social for all the support and guidance!
To say that this took a village would be an understatement. I’m incredibly grateful to my collaborators in the @brangwynnelab.bsky.social : Troy Comi, @sofiquinodoz.bsky.social, @nima-jaberi.bsky.social , Lenny Wiesner and Lifei Jiang for their hard work and expertise, ...
August 20, 2025 at 8:01 PM
To say that this took a village would be an understatement. I’m incredibly grateful to my collaborators in the @brangwynnelab.bsky.social : Troy Comi, @sofiquinodoz.bsky.social, @nima-jaberi.bsky.social , Lenny Wiesner and Lifei Jiang for their hard work and expertise, ...
🌟 Big picture 🌟
✔️ Condensate morphology encodes function
✔️ Deep-Phase translates images into quantitative readouts of drug biochemical potency
✔️ Future applications: phenotypic screens, MoA studies, biomarkers, single-cell heterogeneity detection
(10/10)
✔️ Condensate morphology encodes function
✔️ Deep-Phase translates images into quantitative readouts of drug biochemical potency
✔️ Future applications: phenotypic screens, MoA studies, biomarkers, single-cell heterogeneity detection
(10/10)
August 20, 2025 at 8:01 PM
🌟 Big picture 🌟
✔️ Condensate morphology encodes function
✔️ Deep-Phase translates images into quantitative readouts of drug biochemical potency
✔️ Future applications: phenotypic screens, MoA studies, biomarkers, single-cell heterogeneity detection
(10/10)
✔️ Condensate morphology encodes function
✔️ Deep-Phase translates images into quantitative readouts of drug biochemical potency
✔️ Future applications: phenotypic screens, MoA studies, biomarkers, single-cell heterogeneity detection
(10/10)
Integrating our discoveries, we came up with a new model for nucleolar phase boundary maintenance, in which both the abundance and processing state of rRNA📊 dictate the multiphase arrangement within this fascinating condensate. (9/10)
August 20, 2025 at 8:01 PM
Integrating our discoveries, we came up with a new model for nucleolar phase boundary maintenance, in which both the abundance and processing state of rRNA📊 dictate the multiphase arrangement within this fascinating condensate. (9/10)
These observations led us to hypothesize that sequential cleavage inhibition💊 (invert & push apart DFCs from GC) followed by transcription inhibition💊 (restore DFC-GC interface) could phenocopy the flower 🌸 morphology. This is indeed what we (and Deep-Phase) saw! (8/10)
August 20, 2025 at 8:01 PM
These observations led us to hypothesize that sequential cleavage inhibition💊 (invert & push apart DFCs from GC) followed by transcription inhibition💊 (restore DFC-GC interface) could phenocopy the flower 🌸 morphology. This is indeed what we (and Deep-Phase) saw! (8/10)
We then conduct mechanistic studies 🔎 to find that beyond its recognized role in resolving supercoiling, TOP1 is also involved in rRNA processing! Its inhibition causes rRNA cleavage defects and a drop in transcription of large ribosomal subunit precursors ❌🏭. (7/10)
August 20, 2025 at 8:01 PM
We then conduct mechanistic studies 🔎 to find that beyond its recognized role in resolving supercoiling, TOP1 is also involved in rRNA processing! Its inhibition causes rRNA cleavage defects and a drop in transcription of large ribosomal subunit precursors ❌🏭. (7/10)
To test the utility of Deep-Phase, we conduct a small molecule screen to uncover a unique nucleolar morphology: the "flower" 🌸! This phenotype, where the DFC-GC interface is maintained but inverted, arises specifically upon DNA Topoisomerase I (TOP1) depletion. (6/10)
August 20, 2025 at 8:01 PM
To test the utility of Deep-Phase, we conduct a small molecule screen to uncover a unique nucleolar morphology: the "flower" 🌸! This phenotype, where the DFC-GC interface is maintained but inverted, arises specifically upon DNA Topoisomerase I (TOP1) depletion. (6/10)
Not just nucleoli! We extend Deep-Phase to nuclear splicing speckles✂️ as well as RSV viral cytoplasmic factories🦠, showing that condensate morphology alterations are a quantitative fingerprint of the degree of RNA biochemistry disruptions within them. (5/10)
August 20, 2025 at 8:01 PM
Not just nucleoli! We extend Deep-Phase to nuclear splicing speckles✂️ as well as RSV viral cytoplasmic factories🦠, showing that condensate morphology alterations are a quantitative fingerprint of the degree of RNA biochemistry disruptions within them. (5/10)
In doing so, we measure dose-response curves from images and demonstrate that they match IC50 values from biochemical experiments: nucleolar morphology becomes a quantitative readout of potencies of drugs that alter ribosome biogenesis! 📉➡️📈 (4/10)
August 20, 2025 at 8:01 PM
In doing so, we measure dose-response curves from images and demonstrate that they match IC50 values from biochemical experiments: nucleolar morphology becomes a quantitative readout of potencies of drugs that alter ribosome biogenesis! 📉➡️📈 (4/10)
We developed Deep-Phase, an automated imaging and deep neural network-based framework that accurately tracks these changes over treatment times⏲️ and concentrations📶 directly from images (no hand-picked features).(3/10)
August 20, 2025 at 8:01 PM
We developed Deep-Phase, an automated imaging and deep neural network-based framework that accurately tracks these changes over treatment times⏲️ and concentrations📶 directly from images (no hand-picked features).(3/10)
Cells organize key processes in biomolecular condensates, such as the multiphase nucleolus where ribosomes are made 🏭. Due to its dynamic and compartmentalized nature, small molecule perturbations of this process💊 lead to fast and distinct morphology rearrangements🌀. (2/10)
August 20, 2025 at 8:01 PM
Cells organize key processes in biomolecular condensates, such as the multiphase nucleolus where ribosomes are made 🏭. Due to its dynamic and compartmentalized nature, small molecule perturbations of this process💊 lead to fast and distinct morphology rearrangements🌀. (2/10)