Ana Burgos
@anaburgos.bsky.social
phd student. bioinformatics. single cell technology. brain. aging. killifish.
Reposted by Ana Burgos
Ageing
#TenaLab @cabd-upo-csic.bsky.social @anaburgos.bsky.social (Killifish/ scRNAseq/ Aging)
https://www.biorxiv.org/content/10.1101/2025.03.24.645095
Special Topics
@aburger2009.bsky.social (Data Reporting/ Metadata/ AI)
https://osf.io/preprints/osf/kvn2x (27/34)
#TenaLab @cabd-upo-csic.bsky.social @anaburgos.bsky.social (Killifish/ scRNAseq/ Aging)
https://www.biorxiv.org/content/10.1101/2025.03.24.645095
Special Topics
@aburger2009.bsky.social (Data Reporting/ Metadata/ AI)
https://osf.io/preprints/osf/kvn2x (27/34)
April 9, 2025 at 7:30 PM
Ageing
#TenaLab @cabd-upo-csic.bsky.social @anaburgos.bsky.social (Killifish/ scRNAseq/ Aging)
https://www.biorxiv.org/content/10.1101/2025.03.24.645095
Special Topics
@aburger2009.bsky.social (Data Reporting/ Metadata/ AI)
https://osf.io/preprints/osf/kvn2x (27/34)
#TenaLab @cabd-upo-csic.bsky.social @anaburgos.bsky.social (Killifish/ scRNAseq/ Aging)
https://www.biorxiv.org/content/10.1101/2025.03.24.645095
Special Topics
@aburger2009.bsky.social (Data Reporting/ Metadata/ AI)
https://osf.io/preprints/osf/kvn2x (27/34)
Reposted by Ana Burgos
@obog.bsky.social @anaburgos.bsky.social (Spermatogenesis/ Multiomics/ Epigenetics)
https://www.biorxiv.org/content/10.1101/2025.03.12.642371 (21/34)
https://www.biorxiv.org/content/10.1101/2025.03.12.642371 (21/34)
April 9, 2025 at 7:30 PM
@obog.bsky.social @anaburgos.bsky.social (Spermatogenesis/ Multiomics/ Epigenetics)
https://www.biorxiv.org/content/10.1101/2025.03.12.642371 (21/34)
https://www.biorxiv.org/content/10.1101/2025.03.12.642371 (21/34)
For even more in-depth analyses, check out our preprint! A huge thank you to all who worked on this project— @gndc.bsky.social @jlopezrios.bsky.social #silvianaranjo #vallehernandez #juanjtena #martamoreno #anafernandezmiñan #lorenaardila #alejandrodelosreyes
March 25, 2025 at 5:30 PM
For even more in-depth analyses, check out our preprint! A huge thank you to all who worked on this project— @gndc.bsky.social @jlopezrios.bsky.social #silvianaranjo #vallehernandez #juanjtena #martamoreno #anafernandezmiñan #lorenaardila #alejandrodelosreyes
While killifish is a powerful model for studying aging, extrapolating results to mammals should be done cautiously. Nonetheless, its neotenic brain traits might help identify promising targets for regenerative or anti-aging therapies. 8/9
March 25, 2025 at 5:30 PM
While killifish is a powerful model for studying aging, extrapolating results to mammals should be done cautiously. Nonetheless, its neotenic brain traits might help identify promising targets for regenerative or anti-aging therapies. 8/9
In vertebrates, embryos typically show higher cell proliferation rates compared to adults. Our results suggest that the neotenic traits we observed in the adult killifish brain could support rapid growth and/or help mitigate challenges associated with its high metabolic rate. 7/9
March 25, 2025 at 5:30 PM
In vertebrates, embryos typically show higher cell proliferation rates compared to adults. Our results suggest that the neotenic traits we observed in the adult killifish brain could support rapid growth and/or help mitigate challenges associated with its high metabolic rate. 7/9
To test if embryonic gene expression in adult killifish brains is incidental or a broader trend, we compared our data to zebrafish single-cell profiles from adult brains and embryos. Remarkably, killifish brains aligned more with embryonic profiles. 6/9
March 25, 2025 at 5:30 PM
To test if embryonic gene expression in adult killifish brains is incidental or a broader trend, we compared our data to zebrafish single-cell profiles from adult brains and embryos. Remarkably, killifish brains aligned more with embryonic profiles. 6/9
Our findings are further supported by the identification of primitive hematopoietic progenitor cells (HPC), present in all 7 single-cell adult samples, along with primitive erythrocytes expressing embryonic hemoglobin genes. 5/9
March 25, 2025 at 5:30 PM
Our findings are further supported by the identification of primitive hematopoietic progenitor cells (HPC), present in all 7 single-cell adult samples, along with primitive erythrocytes expressing embryonic hemoglobin genes. 5/9
Surprisingly, we found a fascinating feature: evidence of primitive hematopoiesis in the adult killifish brain. We identified stromal cell clusters (EC & Mural runx1⁺) with mixed hemangioblast/hemogenic traits—similar to rapid transitions observed in embryonic zebrafish. 4/9
March 25, 2025 at 5:30 PM
Surprisingly, we found a fascinating feature: evidence of primitive hematopoiesis in the adult killifish brain. We identified stromal cell clusters (EC & Mural runx1⁺) with mixed hemangioblast/hemogenic traits—similar to rapid transitions observed in embryonic zebrafish. 4/9
We used single-cell RNA-seq to profile brain samples from killifish across their entire adult lifespan—from young adulthood to old age—in both males and females, with the goal of understanding how aging unfolds in the brain. 3/9
March 25, 2025 at 5:30 PM
We used single-cell RNA-seq to profile brain samples from killifish across their entire adult lifespan—from young adulthood to old age—in both males and females, with the goal of understanding how aging unfolds in the brain. 3/9
Killifish is one of the shortest-lived vertebrates on Earth. It inhabits seasonal ponds and has evolved to grow, reproduce, and age within just a few months. Due to its rapid life cycle, it is now widely used as a model to study aging and lifespan regulation in real time. 2/9
March 25, 2025 at 5:30 PM
Killifish is one of the shortest-lived vertebrates on Earth. It inhabits seasonal ponds and has evolved to grow, reproduce, and age within just a few months. Due to its rapid life cycle, it is now widely used as a model to study aging and lifespan regulation in real time. 2/9
Finally, big thanks to everyone involved in this project, your contributions were essential in making this research possible: Fan-Suo G, Gala P, Thirsa B, Estefanía S, María Almuedo, Juan J Tena, @obog.bsky.social @aruizherrera.bsky.social @ageinves.bsky.social @cabd-upo-csic.bsky.social and others
March 13, 2025 at 12:43 PM
Finally, big thanks to everyone involved in this project, your contributions were essential in making this research possible: Fan-Suo G, Gala P, Thirsa B, Estefanía S, María Almuedo, Juan J Tena, @obog.bsky.social @aruizherrera.bsky.social @ageinves.bsky.social @cabd-upo-csic.bsky.social and others
In elongated spermatids, despite chromatin compaction and transcriptional silencing, thousands of sites remain accessible which coincide with CpG islands and which might be retained intergenerationally to aid with early developmental processes in the next generation. 6/7
March 13, 2025 at 12:43 PM
In elongated spermatids, despite chromatin compaction and transcriptional silencing, thousands of sites remain accessible which coincide with CpG islands and which might be retained intergenerationally to aid with early developmental processes in the next generation. 6/7
What about chromatin? scATAC-seq show local and global remodeling. We identify thousands of locus-specific events and track chromatin accessibility rising during spermatogonial differentiation, peaking in spermatocytes, and dropping in spermatids, paralleling txn shutdown. 5/7
March 13, 2025 at 12:43 PM
What about chromatin? scATAC-seq show local and global remodeling. We identify thousands of locus-specific events and track chromatin accessibility rising during spermatogonial differentiation, peaking in spermatocytes, and dropping in spermatids, paralleling txn shutdown. 5/7
Our WGBS data reveal a stable 5mCG landscape across spermatogenesis. However, we detected ~4000 localized changes in spermatocytes that coincide with CpG islands and that might reflect structural or gene-regulatory events associated with these cells. 4/7
March 13, 2025 at 12:43 PM
Our WGBS data reveal a stable 5mCG landscape across spermatogenesis. However, we detected ~4000 localized changes in spermatocytes that coincide with CpG islands and that might reflect structural or gene-regulatory events associated with these cells. 4/7
Next, we wanted to know which genes drive the transformation of spermatogonial cells into mature sperm. Using pseudotime analysis methods, we mapped their differentiation paths and identified ~160 key gene drivers, helping to decode the molecular blueprint of spermatogenesis. 3/7
March 13, 2025 at 12:43 PM
Next, we wanted to know which genes drive the transformation of spermatogonial cells into mature sperm. Using pseudotime analysis methods, we mapped their differentiation paths and identified ~160 key gene drivers, helping to decode the molecular blueprint of spermatogenesis. 3/7
Using single-cell omics (scRNA-seq, scATAC-seq) and WGBS from sorted germ cell populations we mapped the entire journey! Our scRNA-seq data reveal diverse populations ranging from undifferentiated spermatogonia to elongated spermatids. 2/7
March 13, 2025 at 12:43 PM
Using single-cell omics (scRNA-seq, scATAC-seq) and WGBS from sorted germ cell populations we mapped the entire journey! Our scRNA-seq data reveal diverse populations ranging from undifferentiated spermatogonia to elongated spermatids. 2/7
thank you! ☺️ I hope this can help as much ppl as possible ✨
June 20, 2024 at 10:30 AM
thank you! ☺️ I hope this can help as much ppl as possible ✨
🥹♥️ I would love to eliminate the bias when it comes to opportunities for collaboration.
June 20, 2024 at 8:31 AM
🥹♥️ I would love to eliminate the bias when it comes to opportunities for collaboration.