congrats - and great to see some more rhodopsins characterized from the CPR!

thanks for flagging.

@tendersombrero.bsky.social may have some thoughts?

fingers crossed for good data!

cool, im interested - will send you an email!

is research on mesophilic environments eligible ?!

amen. feel like i need a print-out version for my desk, or something!

tested for what, exactly? in any case, there are nice examples from marine micro - HOTS/BATS/ san pedro for long term time series, geotraces malaspina and tara for geographic breadth …

yep

you are correct - thanks for flagging that!

one other quick question for you - seeing lots of otu coverage vals with 0.31 decimal increments - expected behavior? if so, related to default kmer len?

ah, that's great! working mostly with illumina right now but this may be useful in the future. kudos on your documentation, by the way - very clear

great - thanks!

congrats to the team and thanks for the great tool! curious if you have any tips/tricks for 'supplement' - im concerned for ex. that adding more erroneous MAGs from current nanopore chemistry might mess with smafa

this was me using rclone yesterday - why u so slow!?

we love to see it.. also neat they derived their expected breadth calculation from an '88 paper

and then there’s infant gut…

but even without that you could get a sense for % of reads composed by your genome(s) of interest - an imperfect (of course) proxy.

i also love branchwater.jgi.doe.gov but im not sure that will give you rel abund, simply presence across all SRA - a very useful starting point for more detailed work